摘要
目的 探讨自动化磁珠法前处理在液相色谱串联质谱(LC-MS/MS)检测血浆儿茶酚胺及其代谢物中的应用效果.方法 采用自动化磁珠法提取血浆标本中的儿茶酚胺[去甲肾上腺素(NE)、肾上腺素(E)、多巴胺(DA)]及其代谢物[甲氧基去甲肾上腺素(NMN)、甲氧基肾上腺素(MN)、3-甲氧酪胺(3-MT)],并采用LC-MS/MS检测,分析该检测方法的线性、准确度、精密度及基质效应(ME)等性能.结果 NE、E、DA、NMN、MN、3-MT检测的线性相关系数r2均＞0.99,各浓度值与标准值偏差范围为0％～14.84％,均＜15％.NE、E、DA、NMN、MN、3-MT的低、中、高值质控品批内变异系数(CV)为0％～13.480％,批间CV为1.260％～13.590％,符合批内、批间CV均≤15％的要求.NE、E、DA、NMN、MN、3-MT的ME均在-20％～20％范围内,说明ME影响较小.结论 LC-MS/MS检测儿茶酚胺及其代谢物采用自动化磁珠法进行血浆标本前处理,各指标检测的线性、准确度、精密度及ME均符合行业标准.
Abstract
Objective To investigate the effectiveness of automatic magnetic bead pretreatment in the detecting catecholamines and their metabolites in plasma by liquid chromatography tandem mass spectrometry(LC-MS/MS).Methods With the use of Automated magnetic bead method to extract catecholamines[norepinephrine(NE),epinephrine(E),dopamine(DA)]and their metabolites[methoxynorepinephrine(NMN),methoxyepinephrine(MN),3-methoxytyramine(3-MT)]from plasma samples and the use of LC-MS/MS to detect,the linearity,accuracy,precision,and matrix effects(ME)of this detection method were analyzed.Results The linear correlation coefficients r2 of NE,E,DA,NMN,MN and 3-MT detection were greater than 0.99,and the deviation range of each concentration value from the standard value was 0％～14.84％,all of which were less than 15％.The intra-batch coefficient of variation(CV)of low,medium and high values of NE,E,DA,NMN,MN and 3-MT was 0％～13.480％,and the inter-batch CV was 1.260％～13.590％,which met the requirements of both intra-batch and inter-batch CV≤15％.The ME of NE,E,DA,NMN,MN,and 3-MT were all in the range of-20％to 20％,indicating that the effect of ME was small.Conclusion With the use of LC-MS/MS for detecting catecholamines and their metabolites,and the use of automated magnetic bead method for plasma sample pretreatment,the linearity,accuracy,precision,and ME detection results for each indicator met industry standards.
维普
万方数据