In this experiment, a set of differential host of near-isogenic lines (NILs) with different resistance gene was used as materials. SSR primers which were located in Htl, Ht2, Ht3 and HtN nearby were detected for their specific to Ht gene by PCR amplification. We have got four pairs of effective Htl gene labeled primers, seven pairs of effective Ht2 gene labeled primers. But no polymorphism was found in Ht3 gene and HtN gene.
NETL
NSTL
万方数据
维普
