Effects of Endosperm Crack on Protective Enzyme Activities During Maize Seeds Germination
Maize hybrid seeds with endosperm no crack, endosperm single crack, endosperm double crack and endosperm turtle crack were used as test materials. During seed germination, the changes of superoxide dismutase (SOD) activity, peroxidase(POD) activity, catalase(CAT) activity, malondialdehyde(MDA) content and membrane per-meability were studied. The results showed that with the seed germination process forward, the SOD activity, POD ac-tivity, CAT activity and cell membrane permeability were increased. From the point of protective enzyme activity in-creased size, the POD activity was the most, the CAT activity in the second, the SOD activity was the least. And the protective enzyme activity of seeds with endosperm turtle crack was the lowest, the protective enzyme activity of seeds with endosperm no crack was the highest. The enzyme activities of SOD, POD and CAT in seeds with endosperm turtle crack were significantly lower than those of endosperm no crack. The enzyme activities of SOD, POD and CAT in seeds with endosperm double crack were significantly lower than those of endosperm no crack. With the seed germi-nation process forward, the MDA content was decreased. The MDA content of seeds with endosperm turtle crack was significantly higher that than of endosperm no crack. The MDA content of seeds with endosperm double crack was significantly higher that than of endosperm no crack. Conclusions as a result, the endosperm crack had adverse effects on seed germination metabolism, should reduce the endosperm crackles in the seed production.