Discussion about the Purity of Maize Standard Molecular Identification Development
To determine a set of feasible technical standard of the purity molecular identification,the relationship between the purity and uniformity identification was analyzed,and discussed eight problems associated with purity identification standards including whether to cover all variety types,if identifying all miscellaneous plant types,whether asking for parent inbred lines,whether correction with field plot planting appraisal result,whether specified test platform,whether authenticity identification before purity identification,whether all the samples could be identified,whether applying to purity identification of genetically modified varieties.The appraisal primer selection and evaluation procedure of maize hybrid purity molecular identification is established.Four factors impacting the field plot planting appraisal results and molecular identification were analyzed,including weak seedlings,disease seedlings,atypical plants,seed germination rates.