Identification of the Purity of Complex Samples by SSR Multiplexed Reaction System
To address the issue of difficult purity identification in complex maize samples,including genetic in-stability and backcross plants,a comprehensive detection scheme for purity detection in complex samples was devel-oped.This scheme was constructed by analyzing 3,998 national and provincial approved maize varieties,and incor-porating techniques such as rapid extraction and multiple amplification systems.By analyzing 53 samples of maize hybrid Zhengdan958 from different sources,it was found that when using a single locus for purity determination,there were significant differences in results among different loci due to genetic instability.However,when three or more loci were used for comprehensive determination,accurate and stable purity detection results could be ob-tained.The comprehensive detection scheme allows for the generation of multiple SSR marker fingerprints with mini-mal increase in time and cost,using only a few additional primers.This enables a more comprehensive and accurate analysis of complex samples,providing solutions for quality issues in seed production involving complex samples.
MaizeHybridComplex samplePuritySSR markerMultiplexed reaction system
NETL
NSTL
万方数据
