Study on the Induction Efficiency and Doubling Efficiency of Maize with Different Germplasm Bases
There are differences in the induction rate and doubling rate of population constructed by different genetic basis breeding test materials.In order to solve the problem of low induction rate and doubling rate of maize haploid breeding at present,in this study,the backbone inbred lines of different populations PH4CV,Mo 17,PH6WC,Zheng 58,D299,H29,Danhuang 34,Dan598,Chang7-2 and S121 were used as the basic materials.Ac-cording to intra-group and inter-group hybridization,a total of 15 basic populations were assembled,and four in-bred generations of F1,F2,F3 and F4 were formed respectively to study the haploid induction rate and doubling rate.High-frequency inducement line Danyou 3 was used to induce haploid of backbone inbred lines and inbred progeny with different genetic bases,and the haploid seeds of each generation were doubled with colchicine concentration of 0.06％.The results showed that there were significant differences in the induction rate among inbred lines of differ-ent germplasm groups.Lancaster and Reid groups PH4CV,Mo17,PH6WC,Zheng 58 had higher induction rate.The high induction rate groups also had higher induction rate through intra-group and inter-group combination of basic materials.At the same time,the utilization of heterosis improved the induction rate of LRC,SPT and PB groups.The induction rate decreased significantly with the increase of inbred generation,and the best effect was doubled in F2 generation.
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