Genetic Analysis and Gene Mapping of the Male Sterile Mutant ms1153 in Maize
Using the male sterile mutant ms1 153 as the material,the cause of the male sterile phenotype in the mutant were analyzed by the observation of chromosomal behavior and paraffin section of anthers during different stages.The genetic mode of ms1153 and the physical location of MS1153 were determined with F,and F2 popula-tion.Compared with wild type,ms1 153 plants had no obvious difference in vegetative growth stage.However,in re-productive growth stage,ms1 153 anthers could not emerge from the glume and the accumulation of the starch in the mature pollen grains were disappeared.Cytological analysis revealed that the meiotic process was normal and the degradation of tapetal cells was delayed in the mutant.Genetic analysis showed that the ms1153 male sterility was controlled by a single recessive gene.In addition,MS 1153 gene was located between molecular markers 8243 and 8275 on the chromosome 4 through the map-based cloning,with a physical interval of 221.4-226.2 Mb.Further-more,no male fertility genes were reported in this region,indicating that MS 1153 is a novel gene regulating male fer-tility formation in maize.
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