Analysis of COBRA Gene Family and Gene Editing of ZmCOBL03 in Maize
The sequence characteristics,evolutionary relationships with other species and tissue-specific ex-pression patterns of 10 COBRA family members were analyzed by bioinformatics method.The results showed that all the 10 COBRA family genes contained CCVS conserved domains and were localized in the cell membrane.The results of phylogenetic analysis showed that the family could be divided into two subfamilies,and the genes in each subfamily had similar gene structure and physical and chemical properties.Further research focused on the signifi-cant member ZmCOBL03 as a candidate gene for gene editing.In this study,using a dual target site design strategy,maize lines with targeted mutations of the ZmCOBL03 gene were successfully obtained through maize genetic trans-formation mediated by Agrobacterium tumefaciens and the CRISPR/Cas9 gene editing technology.Phenotypic analy-sis confirmed that the mutation of ZmCOBL03 gene seriously affected the normal development of maize,which showed dwarf plants without female and male panicle,and confirmed that ZmCOBL03 gene played an important role in plant cell wall cellulose synthesis.
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