摘要
利用甜玉米自交系SHL03和SHL01为亲本构建的F2∶3群体,用离子色谱法(IC)测定葡萄糖(GLU)、果糖(FRU)、麦芽糖(MAL)和蔗糖(SUC)的含量,用SMS测定果肉硬度(FH)、种皮硬度(PH)、种皮脆性(PB)和果肉紧实度(FM).结果表明,总共定位到与可溶性糖相关的QTL 34个,子粒柔嫩度相关的QTL 28个,位于1、2、3、4、5、6、7、8、10号染色体上,分别解释表型变异的 1％～15.8％.主效QTL qFH3-1、qFH3-2、qFH5-2、qFH5-3、qGLU2-1、qG-LU2-2的表型贡献率分别为12.2％、11.4％、12.1％、10.5％、15.8％和12.8％.在主效QTL的置信区间共找到44个注释基因,这些候选基因和相关的QTL为利用分子标记辅助选择具有更好可溶性糖含量的玉米材料和相关功能基因的克隆提供资源.
Abstract
In this study,the F2∶3 population was constructed using the sweet corn inbred lines SHL03 and SHL01 as parents.The contents of glucose(GLU),fructose(FRU),maltose(MAL)and sucrose(SUC)were determined by ion chromatography(IC),and flesh hardness(FH),pericarp hardness(PH),pericarp brittleness(PB)and firmness(FM)were determined by SMS TA.XT plus texture analyzer.A total of 34 QTL related to soluble sugar,28 QTL relat-ed to grain tenderness,located in chromosome 1,2,3,4,5,6,7,8,and 10,explaining 1％-15.8％of the phenotypic variation,respectively.The phenotypic contribution rates of the major QTLs qFH3-1,qFH3-2,qFH5-2,qFH5-3,qGLU2-1,and qGLU2-2 were 12.2％,11.4％,12.1％,10.5％,15.8％,and 12.8,respectively.Within the confidence intervals of the major QTLs,a total of 44 annotated genes were identified,and these candidate genes and related QTLs provided resources for molecular marker-assisted selection of corn materials with better soluble sugar content and clones of related functional genes.
基金项目
上海市科技兴农项目(沪农科创字[2019]第1-1号)
上海市科技支撑计划(20392000400)
国家玉米产业技术体系建设项目(CARS-02-86)
上海市现代农业产业技术体系建设项目(沪农科产字[2017]第10号)
上海市农科院农业科技创新支撑领域研究专项()
NETL
NSTL
万方数据