QTL Mapping for Soluble Sugar and Kernel Tenderness Traits Using Sweet Corn F2:3 Populations
In this study,the F2∶3 population was constructed using the sweet corn inbred lines SHL03 and SHL01 as parents.The contents of glucose(GLU),fructose(FRU),maltose(MAL)and sucrose(SUC)were determined by ion chromatography(IC),and flesh hardness(FH),pericarp hardness(PH),pericarp brittleness(PB)and firmness(FM)were determined by SMS TA.XT plus texture analyzer.A total of 34 QTL related to soluble sugar,28 QTL relat-ed to grain tenderness,located in chromosome 1,2,3,4,5,6,7,8,and 10,explaining 1％-15.8％of the phenotypic variation,respectively.The phenotypic contribution rates of the major QTLs qFH3-1,qFH3-2,qFH5-2,qFH5-3,qGLU2-1,and qGLU2-2 were 12.2％,11.4％,12.1％,10.5％,15.8％,and 12.8,respectively.Within the confidence intervals of the major QTLs,a total of 44 annotated genes were identified,and these candidate genes and related QTLs provided resources for molecular marker-assisted selection of corn materials with better soluble sugar content and clones of related functional genes.
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