Identification of Maize Nitrogen Stress Responsive NRTGenes and Cloning and Expression of ZmNRT2.5
Nitrate transporters(NRTs)play an important role in NO3-uptake or transporters in plant roots.To in-vestigate the function of NRTs gene in maize nitrogen uptake,seven differentially expressed ZmNRTs genes that re-spond to nitrogen treatment were identified from the transcriptome data.Analysis of promoter cis-acting elements in-dicated that the promoters of these ZmNRTs genes contain multiple plant stress or hormones response elements,sug-gesting that these genes might be involved in abiotic stress or plant hormone regulation of nitrogen uptake of maize.The ZmNRT2.5,which showed the most significant response to nitrogen treatment,was cloned from maize roots.The ZmNRT2.5 CDs of 1 563 bp in length,encoded a 520 amino acid.Phylogenetic analysis revealed that ZmNRT2.5 had the highest homology with AtNRT2.5,containing a conserved nitrate transport domain,in which the secondary structure was composed of α-helix and random coils,including 11 transmembrane domains.Real-time quantitative PCR analysis showed that ZmNRT2.5 was mainly expressed in roots,old leaves,and leaf sheaths.Low nitrogen treat-ment significantly induced the expression of ZmNRT2.5 gene in root,plant hormones abscisic acid,gibberellin and ethylene regulated the expression of ZmNRT2.5.Concurrently,the expression of ZmNRT2.5 was significantly inhib-ited by salt stress.
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万方数据
