玉米氮胁迫响应NRT基因的鉴定及ZmNRT2.5的克隆和表达分析
Identification of Maize Nitrogen Stress Responsive NRTGenes and Cloning and Expression of ZmNRT2.5
王玉斌 1张志贤 1张岱奕 1刘薇 1张彦威1
作者信息
- 1. 山东省农业科学院作物研究所/山东省特色作物工程实验室,济南 250100
- 折叠
摘要
硝酸盐转运蛋白(Nitrate Transporters,NRTs)在植物根系NO3-吸收或转运中发挥重要作用.为探究玉米NRTs基因在氮素吸收中的功能,从前期转录组数据中鉴定出7个响应氮素处理的差异表达ZmNRTs基因.启动子顺式作用元件分析表明,这些ZmNRTs基因的启动子均含有多个植物逆境或激素应答元件,推测他们可能与玉米非生物胁迫应答或植物激素调控氮素吸收相关.从玉米根系中克隆了对氮素处理响应最大的ZmNRT2.5,该基因CDs全长为1 563 bp,编码520个氨基酸.进化树分析结果表明,ZmNRT2.5与AtNRT2.5同源性最高,含有保守的硝酸盐转运结构域,二级结构以α-螺旋和无规则卷曲为主,含11个跨膜结构域.实时荧光定量PCR分析表明,Zm-NRT2.5主要在根、老叶和叶鞘中表达.低氮处理显著诱导ZmNRT2.5在根中的表达,植物激素脱落酸、赤霉素和乙烯均参与调控ZmNRT2.5的表达.同时,ZmNRT2.5基因的表达受到盐胁迫的显著抑制.
Abstract
Nitrate transporters(NRTs)play an important role in NO3-uptake or transporters in plant roots.To in-vestigate the function of NRTs gene in maize nitrogen uptake,seven differentially expressed ZmNRTs genes that re-spond to nitrogen treatment were identified from the transcriptome data.Analysis of promoter cis-acting elements in-dicated that the promoters of these ZmNRTs genes contain multiple plant stress or hormones response elements,sug-gesting that these genes might be involved in abiotic stress or plant hormone regulation of nitrogen uptake of maize.The ZmNRT2.5,which showed the most significant response to nitrogen treatment,was cloned from maize roots.The ZmNRT2.5 CDs of 1 563 bp in length,encoded a 520 amino acid.Phylogenetic analysis revealed that ZmNRT2.5 had the highest homology with AtNRT2.5,containing a conserved nitrate transport domain,in which the secondary structure was composed of α-helix and random coils,including 11 transmembrane domains.Real-time quantitative PCR analysis showed that ZmNRT2.5 was mainly expressed in roots,old leaves,and leaf sheaths.Low nitrogen treat-ment significantly induced the expression of ZmNRT2.5 gene in root,plant hormones abscisic acid,gibberellin and ethylene regulated the expression of ZmNRT2.5.Concurrently,the expression of ZmNRT2.5 was significantly inhib-ited by salt stress.
关键词
玉米/氮胁迫/NRTs/ZmNRT2.5/表达分析Key words
Maize/Nitrogen stress/NRTs/ZmNRT2.5/Expression analysis引用本文复制引用
基金项目
山东省自然科学基金青年基金(ZR2021QC090)
山东省农科院科技创新工程项目(cxgx2022E01)
出版年
2024
NETL
NSTL
万方数据