摘要
利用40对核心SSR标记分析148份玉米自交系的遗传多样性与亲缘关系.结果表明,40对核心SSR标记在148份玉米自交系中共检测出 136个等位变异,每个SSR标记得出的等位变异总数为2～6个,平均3.4个;有效等位基因数(Ne)变幅为1.232 3～5.005 0,平均2.393 9;基因多样性变幅为0.188 5～0.800 2,平均为0.525 2;引物的多态性信息含量(PIC)变幅为0.178 1～0.770 5,平均0.461 7.利用UPGMA聚类法将148份玉米自交系划分为Reid、旅大红骨、PB、Lancaster、塘四平头、中间类群,共6个类群,其中,主要以Reid、旅大红骨、PB、Lancaster和塘四平头这5个类群为主,种群的划分与系谱基本吻合.
Abstract
Forty core SSR markers were used to explore the genetic diversity and genetic relationship of 148 maize inbred lines.The results indicated that,a total of 136 allelic variations were detected across the 148 maize in-bred lines using the 40 core SSR markers,with each marker exhibiting 2 to 6 alleles and an average of 3.4 alleles.The effective number of alleles(Ne)was 1.232 3 to 5.005 0,with an average of 2.393 9.The gene diversity ranged from 0.188 5 to 0.800 2,with an average of 0.525 2.The polymorphic information content(PIC)of SSR markers was between 0.178 1 and 0.770 5,with an average of 0.461 7.By UPGMA cluster analysis method,148 maize inbred lines were divided into 6 major groups,which were named Reid,Luda Red Cob,PB,Lancaster,Sipingtou,and un-certain group.The division of populations largely aligned with the 5 principal groups of Reid,Luda Red Cob,PB,Lancaster,and Sipingtou,reflecting a fundamental agreement with the genealogy.
基金项目
吉林省科技发展计划(20210202005NC)
国家级大学生创新创业训练计划(202210193001)
国家级大学生创新创业训练计划(202210193005)
吉林农业大学本科生科技创新基金(2023)()
吉林省教育厅科学技术研究项目(JJKH20230396KJ)
NETL
NSTL
万方数据