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干旱耐性差异玉米自交系叶面泡状细胞特性分析

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以6种不同干旱耐性玉米自交系为研究对象,开展不同自交系苗期干旱耐性、泡状细胞特性及干旱胁迫后泡状细胞形变等研究,揭示泡状细胞与干旱胁迫耐性差异玉米自交系干旱胁迫前后的特性及反应的关联性,在细胞水平上探索重要农作物干旱胁迫反应机制.结果表明,玉米自交系B73、PH4CV、HiC对干旱胁迫较为敏感,Mo17干旱耐性较强,Zong31、Z58次之.泡状细胞切片显示,叶片表皮泡状细胞簇数随自交系干旱耐性增强而增多,每簇泡状细胞数量随自交系干旱耐性增强而减少.干旱处理后,泡状细胞发生明显形变,自交系泡状细胞形变程度与其干旱耐性、叶片萎蔫程度、红外热成像、组织含水量和膜脂氧化产物MDA(Malondialdehyde)积累等胁迫反应特征呈对应关系,表明玉米叶片泡状细胞在调节植物干旱胁迫反应中发挥重要作用.
Characteristic Analysis of Leaf Bulliform Cells in Maize Inbred Lines with Different Drought Tolerance
Six inbred lines of maize with different drought tolerance were used to investigate the drought toler-ance,characteristics of bulliform cells and the deformation of bulliform cells after drought stress.Meanwhile,the study was focus on revealing the characteristics of bulliform cell before and after drought stress in maize inbred lines with different tolerance to drought stress and exploring the mechanism of drought stress response of crops at cellular level.The results indicated that B73,PH4CV and HiC exhibited higher sensitivity to drought stress,with severe leaf crimping and wilting phenotype after drought treatment,while Mo 17 displayed stronger drought tolerance,followed by Zong31 and Z58.The results of bulliform cell sections showed that the number of bulliform cells clusters in leaf epidermis increased and the number of bulliform cells in each cell cluster decreased in inbred lines with higher drought tolerance.After treatment of drought,the bulliform cells were obviously deformed.And the degree of defor-mation of bulliform cells in inbred lines corresponded to the stress response characteristics such as drought toler-ance,leaf wilting degree,leaf surface temperature,tissue water content and malondialdehyde(MDA)accumulation,indicating that the bulliform cells in maize leaves played an important role in maize drought stress response.

MaizeBulliform cellDrought stressAdaptive response

刘宇欣、刘芦瑶、穆心雨、蒋玲、王喆、韩悦、杨筱荷、李保珠

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河南大学生命科学学院/河南大学农学院/河南大学省部共建作物逆境适应与改良国家重点实验室/棉花生物学国家重点实验室/植物逆境生物学重点实验室,河南开封 475004

河南大学三亚研究院,海南三亚 572025

河南大学高等学科交叉研究院,河南开封 475004

玉米 泡状细胞 干旱胁迫 适应性反应

三亚崖州湾科技城科技专项省部共建作物逆境适应与改良国家重点实验室开放基金(2022)

SCKJ-JYRC-2022-782022KF03

2024

玉米科学
吉林省农业科学院 国家玉米工程技术研究中心(吉林) 国家玉米改良中心 中国农业科技东北创新中心

玉米科学

CSTPCD北大核心
影响因子:1.163
ISSN:1005-0906
年,卷(期):2024.32(2)
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