Screening and Identification of Maize Rhizosphere Growth-Promoting Bacteria and Evaluation of Their Growth-Promoting Effects
To screen excellent maize rhizosphere promoting bacteria,82 rhizosphere promoting bacteria were isolated,purified and identified from the maize rhizosphere soil of the MERIS region of Qiqihar city.The 82 bacteri-al were identified as Agrobacterium,Bacillus,Enterobacter,Klebsiella,Lelliottia,Priestia,Pseudomonas,Sphingomo-nas and Staphylococcus,included 48 phosphate-solubilizing bacteria,12 potassium bacteria,13 free-living nitro-gen-fixer and 9 associative nitrogen-fixer.By measuring the growth-promoting ability,26 strains with stronger abili-ty were screened to verify their growth-promoting effect on maize seedlings.The results showed that most strains had a growth promoting effect on maize seedlings.The plant height of maize seedlings treated with strain P24 could reach 1.18 times of the control;the fresh weight of maize seedlings treated with strain P46 could reach 1.45 times of the control;the root length treated with strain P48 could reach 4.45 times of the control;the number of apices treat-ed with ZN4 could reach 3.84 times of the control;the number of branches of the root treated with P48 could reach 4.38 times of the control.After comprehensive analysis of these five indexes in maize seedlings,it was found that P23,P33,P46,ZN11,P24,and P43 had the highest comprehensive growth promoting ability,significantly increas-ing all indicators of maize seedlings and had a strong development potential.
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