Evaluation of Resistance of Transgenic Cry1A.401 Maize CM8302 and CM8303 to the Ostrinia furnacalis
Through the utilization of agrobacterium mediated method and new insect-resistant gene Cry1A.401,transgenic insect resistant maize CM8302 and CM8303 were developed.Three generations of transgenic materials,BC4F1,BC4F2 and BC4F3 were obtained through backcross screening.The methods of qRT-PCR and ELISA were used to determine that Cry1A.401 gene was expressed in the heartleaves,silks,and grains,and no significant differ-ence in the expression amount of the target gene among three generations.Field test results with insect-artificial in-oculation showed that the resistance level of CM8302 and CM8303 at the heart leaf and the silking stage was high.The results of laboratory bioassay showed that the survival rates of Ostrinia furnacalis larvae on the 6th day after feeding CM8302 and CM8303 heartleaves,silks and grains were 0,4.0％and 4.0％,and 0,4.0％and 2.0％respec-tively.In conclusion,the heartleaves,silks,and grains of transgenic insect-resistant maize CM8302 and CM8303 have significant insecticidal effects and are genetically stable.
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