Photosynthetic Characteristics and Genetic Analysis of the Maize Carbohydrate Distribution Deficient Mutant ygl3
A carbohydrate distribution deficient mutant ygl3 derived from fast neutron radiation with an elite maize line PH6WC was used to determine leaf chlorophyll and carbohydrate content,microscopic observation of chloroplast structure,photosynthetic and genetic characteristics.The results showed that the ygl3 mutant chloro-plast had fewer number and looser morphology,a large amount of starch accumulated in the chloroplasts of vascular bundle sheath,and the irregular arrangement and increased volume of starch particles lead to the expansion of chlo-roplasts and the destruction of membrane structure in comparison with the wild type.The contents of starch,glucose and fructose in the leaves were significantly higher,while the contents of chlorophyll a,chlorophyll b and total chlo-rophyll,and photosynthetic parameters,net photosynthetic rate(Photo),stomatal conductance(Cond)and transpira-tion rate(Trmmol),were significantly lower than those of the wild type,exhibiting typical phenotypic characteristics of carbohydrate allocation defects in maize.Genetic analysis showed that the separation ratio of mutant type(yellow)and wild type(green)in F2 population of ygl3×Mo17 was 1:3,explaining that the yellow mutant was controlled by a single nuclear recessive gene.
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