Study on Agrobacterium-Mediated Transgenic Methods for in Vitro Germination of Pollen Maize
The main methods for maize transgenesis are the Agrobacterium-mediated method and gene gun method.However,both methods require tissue culture,which is demanding and time-consuming,and they are limit-ed by the genotypes of transgenic recipients.In this paper,the promotional hybrid Suyu19 was randomly selected as the material and the introduction of exogenous genes into maize pollen through Agrobacterium infestation of the ger-minating pollen was explored.Then,the female cob was pollinated with the Agrobacterium-infested pollen to obtain transgenic maize.By optimizing the medium formulation,incubation temperature,germination time,Agrobacterium infiltration time,and other technical factors,a new method of Agrobacterium-mediated introduction of exogenous genes into isolated germinating pollen for maize transgenesis has been established.The conversion rate can reach up to 7.27％,and the results of Southern hybridization test showed that the exogenous genes were integrated into the maize genome.The method is exogenous gene introduction has the advantages of simple operation,low cost,and rap-id breeding of new high-yield varieties,providing technical support for maize transgenesis.
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