Cloning and Drought Resistance Function Analysis of Maize Stress Response Gene ZmbZIP15
Twenty-seven bZIP genes involved in the response to drought-rewater stress were screened from transcriptome data,and ZmbZIP15 was found to be at the core node by constructing co-expression network map.The gene is located on chromosome 5,encoding 176 amino acids and contains a highly conserved bZIP domain,which is a hydrophilic protein.The analysis of the protein evolution tree showed that the protein was the closest rela-tive to miscanthus and sorghum,and the furthest relative to barley and wheat.Cis-element analysis of the 2 K pro-moter upstream of ATG of ZmbZIP15 gene revealed multiple binding elements involved in the regulation of abscisic acid,low temperature,and drought.The results from real-time quantitative fluorescence PCR(qRT-PCR)showed that ZmbZIP15 was a constitutive expression gene,which was highly expressed in female panicle and lowest in young stem.Under drought,high temperature,salt and nitrogen stress,the expression of ZmbZIP15 gene was signifi-cantly up regulated,indicating that ZmbZIP15 gene actively participated in and regulated abiotic stress pathways.It was found that overexpression of ZmbZIP15 gene could improve the drought resistance of Arabidopsis thaliana seed-lings under drought stress.Subcellular localization analysis revealed that the protein encoded by this gene is located in the nucleus.
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