Using CRISPR-Cas9 Technique to Edit ZmSh2Gene to Create New Germplasm of Super Sweet Maize
The ZmSh2 gene of maize was selected and CRISPR-Cas9 technology was used to construct the gene editing vector pBUE41 1-ZmSh2.Immature embryos of maize C01 were used as receptors and the target gene was transferred into young embryos of maize by tumefacien-mediated method.Mutant lines with edited ZmSh2 gene were screened using phosphine oxalate as the selector.Phenotypic observation showed that the edited homozygous mutant material was significantly more wrinkled than the control maize inbred line C01.The sweetness of the mu-tant plants measured by a handheld sugar meter reached 25％.The results showed that CRISPR-Cas9 technology can be used to create new germplasm materials of super sweet maize and provide an important theoretical and practi-cal basis for CRISPR-Cas9 to mediate the directed genetic improvement of other traits of maize.
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