Effects of Inoculation Methods and Inoculation Period on Resistance Evaluation of Ear Rot Diseases across Maize Hybrids
This study aimed to evaluate resistance of maize to Fusarium verticillioides ear rot(FER)by compar-ing different inoculation methods and periods using three combinations of maize crosses:high resistance × high resis-tance,high resistance × high susceptible and high susceptible × high susceptible.In the summer of 2022,maize was inoculated at 5 d,10 d and 15 d after silking by kernel injection and two-toothpicks in Shunyi,Beijing and Xinx-iang,Henan,respectively.The results showed that the average FER severity by kernel injection was 6.3％,3.9％and 1.8％higher than that by two-toothpicks method at 5 d,10 d and 15 d after silking,respectively.Inoculation at 5 d after silking,there was no significant difference between different hybrids by kernel injection method,but there was highly significant difference by two-toothpicks method(P＜0.01).Inoculation at 10 d and 15 d after silking showed highly significant differences(P＜0.01)between different hybrids by kernel injection method,but not significant by two-toothpicks method.In conclusion,it is recommended to inoculate at 5 d after silking by the two-toothpick meth-od,while inoculation at 10-15 d after silking by the kernel injection method.
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