This study aimed to evaluate resistance of maize to Fusarium verticillioides ear rot(FER)by compar-ing different inoculation methods and periods using three combinations of maize crosses:high resistance × high resis-tance,high resistance × high susceptible and high susceptible × high susceptible.In the summer of 2022,maize was inoculated at 5 d,10 d and 15 d after silking by kernel injection and two-toothpicks in Shunyi,Beijing and Xinx-iang,Henan,respectively.The results showed that the average FER severity by kernel injection was 6.3％,3.9％and 1.8％higher than that by two-toothpicks method at 5 d,10 d and 15 d after silking,respectively.Inoculation at 5 d after silking,there was no significant difference between different hybrids by kernel injection method,but there was highly significant difference by two-toothpicks method(P＜0.01).Inoculation at 10 d and 15 d after silking showed highly significant differences(P＜0.01)between different hybrids by kernel injection method,but not significant by two-toothpicks method.In conclusion,it is recommended to inoculate at 5 d after silking by the two-toothpick meth-od,while inoculation at 10-15 d after silking by the kernel injection method.
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