Effect of Different Inoculation Methods of Penicillium Ear Rot and the Resistance of New Varieties in Maize
Ear rot is a serious disease of maize,and the incidence of ear rot caused by Penicillium has been in-creasing year by year in China.The development and utilization of resistant cultivars is the most economical,safe,and effective method for controlling ear rot.In this study,the screening of maize resistance to Penicillium ear rot was carried out.Different inoculation methods,including silk spraying,silk channel injection,kernel injection,and two toothpicks method,have been compared for identifying resistance to Penicillium ear rot under different environ-ments over 3 years(2020-2022).The results showed that the kernel injection and toothpick method had moderate disease intensity,which could make different varieties show different levels of resistance responses after artificial in-oculation.The two methods had stable disease,obvious difference in resistance and susceptibility,and accurate identification results.During 2021-2022,7.8％,24.1％,28.6％,24.5％,15.0％of new maize varieties were highly resistant,resistant,moderately resistant,susceptible and highly susceptible to Penicillium ear rot,respectively.These results provide a foundation for understanding the ear rot caused by Penicillium,and should play an impor-tant role in disease prevention.
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