玉米茎腐病致病菌全基因组分泌蛋白预测与分析
Genome-wide Prediction and Analysis of the Secreted Proteins from the Pathogenic Fungus Causing Maize Stalk Rot
曹言勇 1张立立 1李慧敏 1韩胜博 1侯梦薇 1段灿星 2施艳 3张新友1
作者信息
- 1. 河南省农业科学院粮食作物研究所/神农种业实验室,郑州 450002
- 2. 中国农业科学院作物科学研究所,北京 100081
- 3. 河南农业大学植物保护学院,郑州 450002
- 折叠
摘要
九州镰孢菌(Fusarium kyushuense)是一种重要的植物病原菌,可引起玉米茎腐病.为明确九州镰孢菌的分泌蛋白,运用SignalP 6.0、ProtComp 9.0、TMHMM 2.0、big-PI Predictor、TargetP 2.0等软件对九州镰孢菌全基因组12 225条蛋白质序列进行分泌蛋白的预测和筛选.结果表明,九州镰孢菌195全基因组蛋白中共有539条序列具有典型的分泌蛋白特征,占总序列数的4.41％.信号肽-3和-1位置的氨基酸相对保守,其切割位点属于A-X-A型.分泌蛋白功能注释分析结果表明,九州镰孢菌的分泌蛋白中共有264个效应蛋白,其功能主要涉及细胞壁组分降解.对碳水化合物活性酶类(CAZymes)进行预测发现,九州镰孢菌分泌蛋白组中共有168个碳水化合物活性酶,为进一步探究其生物学特性、致病机制和筛选新的镰孢菌致病效应蛋白奠定基础.
Abstract
Fusarium kyushuense,one of the causal agents of maize stalk rot.To investigate the mechanism un-derlying the fungal pathogenicity,secreted proteins of F.kyushuense were predicted by combined using of SignalP 6.0,ProtComp 9.0,TMHMM 2.0,big-PI Predictor and TargetP 2.0 software based on the genome-wide of 12225 protein sequences.In this study,539 secreted proteins were verified,accounting for 4.41％of the total proteins pres-ent in the genome of F.kyushuense.In addition,the amino acids in the position-3 to-1 in the signal peptides were relatively conserved and the signal peptide cleavage site belonged to A-X-A type.The effector prediction results in-dicated that 264 secreted proteins might serve as potential effectors,which mainly involves the degradation of cell wall components.The CAZymes prediction results showed that 168 ones among F.kyushuense putative secreted pro-teins were carbohydrate-active enzymes.Finally,this dissertation provides some theoretical foundation to promote maize resistance breeding and disease management.
关键词
玉米/九州镰孢菌/生物信息学/分泌蛋白Key words
Maize/Fusarium kyushuense/Bioinformatic/Signal peptide引用本文复制引用
基金项目
河南省农科院自主创新基金(2023ZC010)
国家重点研发计划(2021YFD1200700)
出版年
2024
NETL
NSTL
万方数据