玉米自交系Y423萌动胚诱导愈伤组织及愈伤组织再分化的体系建立
Establishment of a Stable Regeneration System Depending on Calli Induced from the Germinating Embryos of Maize Inbred Line Y423
单晓辉 1魏慧慧 1刘洪魁 1董海霄 1李世鹏 1苏胜忠 1原亚萍1
作者信息
- 1. 吉林大学植物科学学院,长春 130062
- 折叠
摘要
幼胚是目前广泛用于玉米组培再生和遗传转化的主要外植体材料,但获取幼胚的数量和质量会受到季节和气候条件的严重影响.通过将玉米子粒在4℃条件下浸泡3 d获得萌动胚,进而以其为外植体,建立玉米组培再生体系.通过调整培养基组成和激素配比,萌动胚在2,4-D浓度为8mg/L的诱导培养基上,初代愈伤组织发生率可达到54.17％,经继代培养胚性愈伤诱导率可达42.13％.胚性愈伤组织在激素组合为1.5 mg/L6-BA+0.1 mg/L NAA的分化培养基上分化率超过40％,经生根培养后可100％生根.基因表达分析同时发现,与体细胞发生相关的7个基因在不同培养阶段的表达变化与胚性愈伤组织的形成密切相关.结果表明,玉米萌动胚可替代幼胚或作为其补充用于玉米组培再生和遗传转化相关工作.
Abstract
Immature embryos are commonly used as the primary explant materials for maize genetic transforma-tion and tissue culture regeneration.However,the quantity and quality of immature embryos can be severely influ-enced by seasonal and climatic conditions.In this study,maize seeds were soaked at 4℃ for three days to obtain ger-minating embryos,which were then used as explants to establish a maize tissue culture regeneration system.By ad-justing the composition of the medium and the hormones ratio,the incubation rate of primary callus could reach 54.17％,and the induction rate of the embryogenic calli could reach 42.13％through successive cultivation on the induction medium with 2,4-D concentration of 8 mg/L.Embryogenic calli were differentiated by more than 40％on the combination of 1.5mg/L 6-BA+0.1 mg/L NAA,and achieved a 100％rooting rate after rooting culture.Gene ex-pression analysis also revealed significant changes in the expression of seven genes associated with somatic embryo-genesis at different stages of culture,closely correlating with the formation of embryogenic calli.These results sug-gest that maize germinating embryos can be effectively utilized as an alternative or supplement to immature embryos for maize tissue culture regeneration and genetic transformation.
关键词
玉米/萌动胚/外植体/组织培养/基因表达Key words
Maize/Germinating embryo/Explant/Tissue culture/Gene expression引用本文复制引用
基金项目
吉林省科技发展计划国际科技合作项目(20200801070GH)
出版年
2024
NETL
NSTL
万方数据