Establishment of a Stable Regeneration System Depending on Calli Induced from the Germinating Embryos of Maize Inbred Line Y423
Immature embryos are commonly used as the primary explant materials for maize genetic transforma-tion and tissue culture regeneration.However,the quantity and quality of immature embryos can be severely influ-enced by seasonal and climatic conditions.In this study,maize seeds were soaked at 4℃ for three days to obtain ger-minating embryos,which were then used as explants to establish a maize tissue culture regeneration system.By ad-justing the composition of the medium and the hormones ratio,the incubation rate of primary callus could reach 54.17％,and the induction rate of the embryogenic calli could reach 42.13％through successive cultivation on the induction medium with 2,4-D concentration of 8 mg/L.Embryogenic calli were differentiated by more than 40％on the combination of 1.5mg/L 6-BA+0.1 mg/L NAA,and achieved a 100％rooting rate after rooting culture.Gene ex-pression analysis also revealed significant changes in the expression of seven genes associated with somatic embryo-genesis at different stages of culture,closely correlating with the formation of embryogenic calli.These results sug-gest that maize germinating embryos can be effectively utilized as an alternative or supplement to immature embryos for maize tissue culture regeneration and genetic transformation.
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