首页|玉米LysM基因家族的全基因组鉴定及表达模式分析

玉米LysM基因家族的全基因组鉴定及表达模式分析

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为揭示LysM基因家族在玉米生长发育及抗病中的作用,利用生物信息学方法对玉米LysM基因家族成员进行鉴定,并对其蛋白理化性质、亚细胞定位、系统发育关系、基因启动子区的顺式作用元件、组织表达以及大斑病菌侵染过程中的表达模式进行分析.结果表明,在玉米中共存在11个ZmLysM家族成员.通过生物信息学分析发现,ZmLysMs蛋白在质膜、叶绿体、胞外均有分布;ZmLysMs成员与单子叶植物水稻和小麦具有较近的亲缘关系;ZmLysMs成员的启动子序列存在多个激素响应、逆境胁迫响应和光响应元件;10个ZmLysMs成员在不同组织中表达,其中ZmLysM1、ZmLysM4和ZmLysM6呈组成性表达模式,ZmLysM10只在根组织中表达.ZmLysM2、ZmLysM6和ZmLysM7在玉米大斑病菌侵染后差异表达,推测其可能在响应大斑病菌侵染过程中发挥作用.
Identification and Expression Pattern Analysis of LysM Gene Family in Maize
To elucidate the role of the LysM gene family in maize growth,development and disease resistance,the members of the LysM gene family in maize were identified using bioinformatics methods,and the physicochemi-cal properties of the proteins,subcellular localization,phylogenetic relationships,cis-acting elements in the promot-er region of the genes,expression patterns in different tissues and in response to Setosphearia turcica infection were also analyzed.The results showed that there were 11 ZmLysM family members in maize.Bioinformatics analysis re-vealed that ZmLysMs proteins were distributed in the plasma membrane,chloroplast,and extracellular.ZmLysMs members were closely related to rice and wheat which were belong to monocotyledons.Several hormone responsive,stress responsive and light responsive elements were identified in the promoter sequences of ZmLysMs members.Furthermore,it was found that 10 ZmLysMs members were expressed in various tissues.Among these,ZmLysM1,ZmLysM4 and ZmLysM6 exhibited a constitutive expression pattern,while ZmLysM 10 was only expressed in root tis-sue.ZmLysM2,ZmLysM6,and ZmLysM7 were differentially expressed after inoculation with S.turcica,indicating a potential role in the response to S.turcica infection.

MaizeLysMDevelopmentStressExpression pattern

周贺、孟岩、尤龙腾、魏淑珍、巩校东、谷守芹、刘玉卫

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河北农业大学生命科学学院/河北省农业微生物生物信息利用技术创新中心,河北保定 071001

衡水学院/河北省湿地生态与保护重点实验室/河北省湿地保护与绿色发展协同创新中心,河北衡水 053010

玉米 LvsM 发育 胁迫 表达模式

国家自然科学基金项目河北省省属高校基本科研业务费研究项目河北省中央引导地方科技发展资金项目现代农业产业技术体系专项

31671983KY2021042216Z2902GCARS-02

2024

玉米科学
吉林省农业科学院 国家玉米工程技术研究中心(吉林) 国家玉米改良中心 中国农业科技东北创新中心

玉米科学

CSTPCD北大核心
影响因子:1.163
ISSN:1005-0906
年,卷(期):2024.32(5)
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