Unlocking the QTL loci to control the tassel branch number of maize is of great significance for maize variety improvement.In this study,310 recombinant inbred lines(RIL)populations derived from the cross be-tween PD80 and PHJ65 were used.The QTL for TBN were mapped using inclusive composite interval mapping(ICIM)based on a genetic map composed of 8384 SNP markers.Based on ICIM,a total of 11 QTLs were detected us-ing phenotypic data in three single environments and best linear unbiased prediction(BLUP),which were distributed on chromosomes 1,2,4,5,7 and 8,with the LOD score ranged from 4.99-11.42,and a phenotypic variation contri-bution rate of 4.62％-11.80％.Five QTLs(qTBN1.1,qTBN2.1,qTBN4,qTBN5,qTBN7)were detected repeatedly under more than two single environments and BLUP values.Among them,qTBN 1.1 and qTBN5 had phenotypic con-tributions greater than 10％and were stable main effect QTLs with physical intervals located at 303.01-304.87 Mb(B73_RefGen_v4_genomic)on chromosome 1 and 168.16-170.97 Mb on chromosome 5.Furthermore,based on bio-informatics analysis and gene function annotation,19 candidate genes with high expression were selected from two major QTL sites.
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