QTL Mapping of the Tassel Branch Number Based on a High-Density Map in Maize
Unlocking the QTL loci to control the tassel branch number of maize is of great significance for maize variety improvement.In this study,310 recombinant inbred lines(RIL)populations derived from the cross be-tween PD80 and PHJ65 were used.The QTL for TBN were mapped using inclusive composite interval mapping(ICIM)based on a genetic map composed of 8384 SNP markers.Based on ICIM,a total of 11 QTLs were detected us-ing phenotypic data in three single environments and best linear unbiased prediction(BLUP),which were distributed on chromosomes 1,2,4,5,7 and 8,with the LOD score ranged from 4.99-11.42,and a phenotypic variation contri-bution rate of 4.62％-11.80％.Five QTLs(qTBN1.1,qTBN2.1,qTBN4,qTBN5,qTBN7)were detected repeatedly under more than two single environments and BLUP values.Among them,qTBN 1.1 and qTBN5 had phenotypic con-tributions greater than 10％and were stable main effect QTLs with physical intervals located at 303.01-304.87 Mb(B73_RefGen_v4_genomic)on chromosome 1 and 168.16-170.97 Mb on chromosome 5.Furthermore,based on bio-informatics analysis and gene function annotation,19 candidate genes with high expression were selected from two major QTL sites.
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