Effects of Coupling Tillage and Planting Patterns on Soil Bacterial Community Structure and Diversity
Through a 4-year localization experiment,the effects of tillage and planting pattern coupling on bac-terial community structure and diversity in farmland soil were investigated using 16S rRNA high-throughput se-quencing technology.The results showed that no-tillage significantly increased the contents of soil organic carbon,total nitrogen,total phosphorus,and available phosphorus.A total of 2 736 OUTs were obtained by OUT clustering,which were divided into 40 phyla,106 classes,230 orders,374 families and 810 genera by annotation.At the phy-lum level,Proteobacteria,Bacteroidetes,Blastomonas and actinomyces were the dominant phyla in farmland soil,and the no-tillage treatment was generally higher than the traditional tillage treatment.The diversity analysis showed that no-till wheat/maize intercropping significantly reduced soil bacterial richness and diversity.Correlation analysis indicated that soil bacterial community structure was mainly affected by soil organic carbon,total nitrogen,available phosphorus,and nitrate nitrogen contents.In conclusion,from the perspective of soil bacterial community composition,no-till tillage is superior to traditional tillage.
MaizeSoil microorganismSoil bacterial communityPhysical and chemical properties of soilCrop rotation
NETL
NSTL
万方数据
