摘要
利用Ht单基因鉴别寄主对2021年采自我国10个省(自治区)57个市县的127株玉米大斑病菌进行生理小种鉴定,采用特异性引物对供试菌株进行交配型检测.生理小种鉴定结果显示,我国玉米大斑病菌主要包括0、1、2、12、3、13、23、N、1N、2N、3N、13N、23N、123N等14个生理小种,其中,0号和1号生理小种分别占供试菌株的34.65％和28.35％,为主要小种类群.我国玉米大斑病菌生理小种存在多元化,东北地区生理分化更为明显.供试菌株对Ht1、Ht2、Ht3、HtN的毒性频率分别为41.73％、9.45％、25.20％、17.32％.交配型检测结果显示,分离株中共有A、a两种交配型,且a交配型比例明显高于A交配型,不同省份玉米大斑病菌交配型组成情况存在差异.
Abstract
The physiological races of 127 isolates of Setosphaeria turcica collected from 57 cities and counties of 10 provinces(autonomous regions)in 2021 were identified on differential hosts containing Ht single resistance gene,and the mating type of the tested isolates was detected by specific primers.The identification results of physio-logical races showed that there were 14 physiological races including 0,1,2,12,3,13,23,N,1N,2N,3N,13N,23N,123N.Among these races,race 0 and race 1 accounted for 34.65％and 28.35％of the tested isolates respec-tively,and were the main race group.There was a diversity of physiological races of S.turcica in China,with more obvious physiological differentiation in Northeast China.The toxicity frequency of the tested isolates to Htl,Ht2,Ht3 and HtN was 41.73％,9.45％,25.20％and 17.32％,respectively.The results of mating type detection showed that there were two mating types,A and a,in the isolates,and the proportion of mating type a was significantly high-er than that of mating type A,and the composition of mating types varied in different provinces.
基金项目
国家重点研发计划课题项目(2018YFD0300307)
国家重点研发计划课题项目(2017YFD0300704)
国家重点研发计划课题项目(2016YFD0300704)
山西农业大学生物育种工程项目(YZGC064)
山西省玉米种业创新良种联合攻关项目()
NETL
NSTL
万方数据