首页|玉米秸秆降解复配菌的构建及其协同效果评价

玉米秸秆降解复配菌的构建及其协同效果评价

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针对秸秆木质纤维素组成结构复杂性,充分利用不同菌株间的协同效应提升秸秆降解效率.以功能单菌株 Achromobacter deleyi strain LMG 3458(A3)、Pseudomonas plecoglossicida strain NBRC(A4)、Aspergillus terreus(A)、Phanerochaete Chrysosporium(P)为试材,通过相互组配构建菌株丰度为1～4的多个复配菌,采用相关性分析和方差分析等方法探明菌株丰度对秸秆降解效率及其生物量的影响.结果表明,秸秆及木质纤维素降解率和酶活性均与复配菌组成丰度呈显著正相关关系,丰度为4时(A3+A4+A+P),其玉米秸秆、纤维素、半纤维素和木质素降解率分别为37.01％、36.27％、33.50％和31.27％,滤纸酶活性、木聚糖酶活性和漆酶活性分别为15.47、5.84和58.05 U/L,表明菌株间以及酶之间存在多样性效应.复配菌组成菌株间具有协同效应,促进秸秆高效降解,具有良好的应用前景.
Construction and Evaluation Synergistic Effect of Corn Straw Degradation Microbial Compound
In view of the complexity of lignocellulosic composition of straw,the synergistic effect between differ-ent strains was fully utilized to improve the degradation efficiency of straw.The functional strains Achromobacter de-leyi strain LMG 3458(A3),Pseudomonas plecoglossicida strain NBRC(A4),Aspergillus terreus(A),Phanerochaete Chrysosporium(P)were used as test materials to obtain multiple compound microbial communities with a strain rich-ness of 1-4,and via correlation analysis and variance analysis to explore the effect of strain richness on straw degra-dation efficiency and biomass.The corn straw and lignocellulose degradation ratio and enzyme activity were positive-ly correlated with the composition abundance of the compound strains.The corn straw,cellulose,hemicellulose and lignin degradation ratio of compound microbial consortia(A3+A4+A+P)were 37.01％,36.27％,33.50％,and 31.27％,respectively,and the activities of filter paper enzyme,xylanase and laccase were 15.47,5.84 U/mL,and 58.05 U/L,respectively.The results showed that there were diversity effects among strains and enzymes.There was synergistic effect among the strains composed of the microbial consortia,which promotes the efficient degradation of straw.Therefore,these findings anticipate promising application prospects for the efficient degradation of straw through microbial consortia.

CornMicrobial consortiaStraw degradationDiversity effectSampling effect

青格尔、张必周、王莉杰、赵文山、于晓芳、高聚林

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内蒙古农业大学/玉米秸秆原位还田微生物内蒙古自治区工程研究中心/内蒙古自治区作物栽培与遗传改良重点实验室,呼和浩特 010019

内蒙古自治区农牧业科学院,呼和浩特 010010

玉米:复配菌 秸秆降解 多样性效应 抽样性效应

国家重点研发计划项目国家重点研发计划项目国家自然科学基金项目国家自然科学基金项目内蒙古自治区高等学校碳达峰碳中和研究专项项目内蒙古自治区科技计划项目内蒙古自治区直属高校基本科研业务费项目国家玉米产业技术体系项目

2023YFD23018012023YFD1501500-013226053231760353STZX2023042023KYPT0023BR22-11-07CARS-02-74

2024

10.13597/j.cnki.maize.science.20240606

玉米科学
吉林省农业科学院 国家玉米工程技术研究中心(吉林) 国家玉米改良中心 中国农业科技东北创新中心

玉米科学

CSTPCD北大核心
影响因子：1.163
ISSN：1005-0906
年,卷(期)：2024.32(6)
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