首页|云南省昆明市玉米白斑病病原菌鉴定及致病性测定

云南省昆明市玉米白斑病病原菌鉴定及致病性测定

扫码查看
采用组织分离、活体接种、形态学和分子生物学鉴定方法,对云南省昆明市玉米白斑病病叶进行病原菌分离、致病性测定及种类鉴定.结果分离得到15株菌株,其中,9株为致病菌,菌株SL-56-2、JX-E466-15和JX-E600-1的致病性最强,发病最快,使玉米叶片产生典型白斑症状;JX-E466-13、JX-E466-12和SL-547-3使玉米叶片产生黄白色小病斑;JX-E102-1、SL-24-8和SL-24-3产生稍大黄白色病斑.形态学及系统发育树结果显示,SL-56-2、JX-E466-15 和 JX-E600-1 为 Epicoccum sorghinum,JX-E102-1、SL-24-8 和 SL-24-3 为E.dendrobii,JX-E466-13、JX-E466-12和SL-547-3为附球菌属未定种.研究表明,E.sorghinum为昆明市玉米白斑病的优势致病种,由E.dendrobii引起的玉米白斑病属首次报道.
Identification and Pathogenicity of the Pathogens of Maize White Spot in Kunming City of Yunnan Province
Using tissue separation,inoculation in vivo,morphological and molecular study methods,the patho-gens of maize white spot in Kunming city of Yunnan province were isolated,the pathogenicity of the isolates were tested and the pathogens were identified,respectively.The results showed that,15 strains were isolated,wherein 9 strains were pathogens.In addition,SL-56-2,JX-E466-15 and JX-E600-1 showed the strongest pathogenicity,with the fastest onset,which could produce typical white spot symptoms on maize leaves.Except that,JX-E466-13,JX-E466-12 and SL-547-3 could produce small yellowish white and JX-E102-1,SL-24-8 and SL-24-3 could produce slightly large yellowish white spots on maize leaves.Based on morphological characteristics and a phyloge-netic tree analyzed,the results showed that,SL-56-2,JX-E466-15 and JX-E600-1 were identified to be Epicoc-cum sorghinum,JX-E102-1,SL-24-8 and SL-24-3 were identified to be E.dendrobii,JX-E466-13,JX-E466-12 and SL-547-3 were identified to be undetermined specie of Epicoccum Link.These results indicated that E.sorghinum to be the dominant one and E.dendrobii to be a new pathogen reported firstly.

MaizeE.sorghinumE.dendrobiiPathogenicity testMorphologyMolecular identification

赵玳琳、吴石平、何海永、吴迅、谭清群、李继业、陈泽辉

展开 >

贵州省农业科学院植物保护研究所,贵阳 550006

贵州省农业科学院旱粮研究所,贵阳 550006

玉米 E.sorghinum E.dendrobii 致病性测定 形态学 分子鉴定

贵州省高层次创新型人才项目2022年度贵州省科技支撑计划生物育种先导性研究项目

[2018]5629

2024

玉米科学
吉林省农业科学院 国家玉米工程技术研究中心(吉林) 国家玉米改良中心 中国农业科技东北创新中心

玉米科学

CSTPCD北大核心
影响因子:1.163
ISSN:1005-0906
年,卷(期):2024.32(6)
  • 2
  • 6