云南省昆明市玉米白斑病病原菌鉴定及致病性测定
Identification and Pathogenicity of the Pathogens of Maize White Spot in Kunming City of Yunnan Province
赵玳琳 1吴石平 1何海永 1吴迅 2谭清群 1李继业 1陈泽辉2
作者信息
- 1. 贵州省农业科学院植物保护研究所,贵阳 550006
- 2. 贵州省农业科学院旱粮研究所,贵阳 550006
- 折叠
摘要
采用组织分离、活体接种、形态学和分子生物学鉴定方法,对云南省昆明市玉米白斑病病叶进行病原菌分离、致病性测定及种类鉴定.结果分离得到15株菌株,其中,9株为致病菌,菌株SL-56-2、JX-E466-15和JX-E600-1的致病性最强,发病最快,使玉米叶片产生典型白斑症状;JX-E466-13、JX-E466-12和SL-547-3使玉米叶片产生黄白色小病斑;JX-E102-1、SL-24-8和SL-24-3产生稍大黄白色病斑.形态学及系统发育树结果显示,SL-56-2、JX-E466-15 和 JX-E600-1 为 Epicoccum sorghinum,JX-E102-1、SL-24-8 和 SL-24-3 为E.dendrobii,JX-E466-13、JX-E466-12和SL-547-3为附球菌属未定种.研究表明,E.sorghinum为昆明市玉米白斑病的优势致病种,由E.dendrobii引起的玉米白斑病属首次报道.
Abstract
Using tissue separation,inoculation in vivo,morphological and molecular study methods,the patho-gens of maize white spot in Kunming city of Yunnan province were isolated,the pathogenicity of the isolates were tested and the pathogens were identified,respectively.The results showed that,15 strains were isolated,wherein 9 strains were pathogens.In addition,SL-56-2,JX-E466-15 and JX-E600-1 showed the strongest pathogenicity,with the fastest onset,which could produce typical white spot symptoms on maize leaves.Except that,JX-E466-13,JX-E466-12 and SL-547-3 could produce small yellowish white and JX-E102-1,SL-24-8 and SL-24-3 could produce slightly large yellowish white spots on maize leaves.Based on morphological characteristics and a phyloge-netic tree analyzed,the results showed that,SL-56-2,JX-E466-15 and JX-E600-1 were identified to be Epicoc-cum sorghinum,JX-E102-1,SL-24-8 and SL-24-3 were identified to be E.dendrobii,JX-E466-13,JX-E466-12 and SL-547-3 were identified to be undetermined specie of Epicoccum Link.These results indicated that E.sorghinum to be the dominant one and E.dendrobii to be a new pathogen reported firstly.
关键词
玉米/E.sorghinum/E.dendrobii/致病性测定/形态学/分子鉴定Key words
Maize/E.sorghinum/E.dendrobii/Pathogenicity test/Morphology/Molecular identification引用本文复制引用
基金项目
贵州省高层次创新型人才项目([2018]5629)
2022年度贵州省科技支撑计划生物育种先导性研究项目()
出版年
2024
NETL
NSTL
万方数据