Identification and Pathogenicity of the Pathogens of Maize White Spot in Kunming City of Yunnan Province
Using tissue separation,inoculation in vivo,morphological and molecular study methods,the patho-gens of maize white spot in Kunming city of Yunnan province were isolated,the pathogenicity of the isolates were tested and the pathogens were identified,respectively.The results showed that,15 strains were isolated,wherein 9 strains were pathogens.In addition,SL-56-2,JX-E466-15 and JX-E600-1 showed the strongest pathogenicity,with the fastest onset,which could produce typical white spot symptoms on maize leaves.Except that,JX-E466-13,JX-E466-12 and SL-547-3 could produce small yellowish white and JX-E102-1,SL-24-8 and SL-24-3 could produce slightly large yellowish white spots on maize leaves.Based on morphological characteristics and a phyloge-netic tree analyzed,the results showed that,SL-56-2,JX-E466-15 and JX-E600-1 were identified to be Epicoc-cum sorghinum,JX-E102-1,SL-24-8 and SL-24-3 were identified to be E.dendrobii,JX-E466-13,JX-E466-12 and SL-547-3 were identified to be undetermined specie of Epicoccum Link.These results indicated that E.sorghinum to be the dominant one and E.dendrobii to be a new pathogen reported firstly.