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连作对玉米根际土壤细菌群落结构的影响

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为探究连作对玉米根际土壤细菌群落特征的影响,应用高通量测序技术对不同连作年限下的玉米根际土壤细菌群落进行测序.结果表明,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、拟杆菌门(Bacteroidetes)、芽单胞菌门(Gemmatimonadetes)等9个门为玉米连作根际土壤细菌的优势类群.随着连作年限的延长,酸杆菌门(Acidobacteria)、浮霉菌门(Planctomycetes)、芽单胞菌 门(Gemmatimonadetes)和棒状杆菌门(Rokubacteria)的丰度先下降后上升,放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)的丰度先上升后下降.其中,变形菌门(Proteobacteria)、芽单胞菌门(Gemmatimonadetes)和棒状杆菌门(Rokubacteria)在连作11年的玉米根际土壤中丰度最低,放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)在连作11年的土壤中丰度最高.土壤pH值、速效钾和有效磷显著影响土壤细菌群落结构,土壤pH值对细菌群落结构的影响最大.
Effects of Continuous Cropping Years on Bacterial Community Structure in Maize Rhizosphere Soil
To investigate the effects of continuous cropping years on bacterial diversity in maize rhizosphere soil,High throughput sequencing technology was used to sequence the rhizosphere soil bacterial communities of maize under different years of continuous cropping.The results showed that,Proteobacteria,Acidobacteria,Gemma-timonadetes were the dominant bacterial groups.Acidobacteria,Planctomycetes,Gemmatimonadetes and Rokubac-teria decreased first and then increased with the extension of continuous cropping years.Actinobacteria,Proteobac-teria,Chloroflexi,Firmicutes and Bacteroidetes showed a trend of first increase and then decrease with the extension of continuous cropping years.Proteobacteria,Gemmatimonadetes and Rokubacteria had the lowest content in maize rhizosphere soil after 11 years of continuous cropping,Actinobacteria,Chloroflexi,Bacteroidetes and Firmicutes all had the highest concentrations in soil after 11 years of continuous cropping.Soil pH,available potassium,and avail-able phosphorus significantly influence the structure of soil bacterial communities,with soil pH having the greatest impact on bacterial community structure.

MaizeContinuous croppingHigh-throughput sequencingBacteria

常浩、徐志鹏、李文学、栾倩倩、周琦、马金慧、汪亮芳、吴之涛、杨克泽、任宝仓

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甘肃省农业工程技术研究院/甘肃省玉米病虫害绿色防控工程研究中心,兰州 730010

武威市玉米病虫害绿色防控技术创新中心,甘肃武威 733006

玉米 连作 高通量测序 细菌

甘肃省陇原青年创新创业人才(个人)项目甘肃省科技计划-乡村振兴专题武威市市级科技计划(重点研发计划)项目甘肃省农业工程技术研究院创新基金项目

2021LQGR0723CXNH0008WW2202YFN004GNG2022ZD04

2024

玉米科学
吉林省农业科学院 国家玉米工程技术研究中心(吉林) 国家玉米改良中心 中国农业科技东北创新中心

玉米科学

CSTPCD北大核心
影响因子:1.163
ISSN:1005-0906
年,卷(期):2024.32(6)
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