摘要
为探究连作对玉米根际土壤细菌群落特征的影响,应用高通量测序技术对不同连作年限下的玉米根际土壤细菌群落进行测序.结果表明,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、拟杆菌门(Bacteroidetes)、芽单胞菌门(Gemmatimonadetes)等9个门为玉米连作根际土壤细菌的优势类群.随着连作年限的延长,酸杆菌门(Acidobacteria)、浮霉菌门(Planctomycetes)、芽单胞菌 门(Gemmatimonadetes)和棒状杆菌门(Rokubacteria)的丰度先下降后上升,放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)的丰度先上升后下降.其中,变形菌门(Proteobacteria)、芽单胞菌门(Gemmatimonadetes)和棒状杆菌门(Rokubacteria)在连作11年的玉米根际土壤中丰度最低,放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)在连作11年的土壤中丰度最高.土壤pH值、速效钾和有效磷显著影响土壤细菌群落结构,土壤pH值对细菌群落结构的影响最大.
Abstract
To investigate the effects of continuous cropping years on bacterial diversity in maize rhizosphere soil,High throughput sequencing technology was used to sequence the rhizosphere soil bacterial communities of maize under different years of continuous cropping.The results showed that,Proteobacteria,Acidobacteria,Gemma-timonadetes were the dominant bacterial groups.Acidobacteria,Planctomycetes,Gemmatimonadetes and Rokubac-teria decreased first and then increased with the extension of continuous cropping years.Actinobacteria,Proteobac-teria,Chloroflexi,Firmicutes and Bacteroidetes showed a trend of first increase and then decrease with the extension of continuous cropping years.Proteobacteria,Gemmatimonadetes and Rokubacteria had the lowest content in maize rhizosphere soil after 11 years of continuous cropping,Actinobacteria,Chloroflexi,Bacteroidetes and Firmicutes all had the highest concentrations in soil after 11 years of continuous cropping.Soil pH,available potassium,and avail-able phosphorus significantly influence the structure of soil bacterial communities,with soil pH having the greatest impact on bacterial community structure.
基金项目
甘肃省陇原青年创新创业人才(个人)项目(2021LQGR07)
甘肃省科技计划-乡村振兴专题(23CXNH0008)
武威市市级科技计划(重点研发计划)项目(WW2202YFN004)
甘肃省农业工程技术研究院创新基金项目(GNG2022ZD04)
NETL
NSTL
万方数据