Developing a Maize Thermosensitive Genic Male Sterile Mutant by the Technology of CRISPR/Cas9
CRISPR/Cas9 technology was used to edit the gene GRMZM2G451028,which is specifically ex-pressed in pollen and encodes a pectin methylesterase inhibitor,and resulting in a frame shift mutant(named pms1)with a 2 bp deletion at 322-323 bp in the coding region of the gene.Compared with the wild-type plants,both the pollen shedding ability and pollen fertility in the homozygous pms1 plants were significantly reduced in spring and autumn.In addition,pollen fertility in the homozygous pms1 under high temperature condition of autumn was signifi-cantly lower than that in spring.Pollen fertility in the heterozygous pms1 showed no significant difference compared to wild-type plants,indicating that the homozygous pms1 is sensitive to temperature.There were no significant dif-ferences in plant height,ear height,ear length and diameter between homozygous pms1 plants and wild-type plants.However,the number of tassel branches and the days from sowing to flowering or silking showed significant differ-ences.
MaizeCRISPR/Cas9Thermosensitive genic male sterilePollen fertility
万方数据
维普
