Comparative Transcriptomics Analysis of the Mechanism of Difference Based on Drought Tolerance in Maize Overexpressing miRNA
A drought-related miRNA was determined by bioinformatics methods,and transgenic maize overex-pressing miR190 was generated.Phenotypic analysis under drought conditions revealed that maize plants overex-pressing miR190 were more sensitive to drought compared to control plants.At the same time,the MDA content of miR190 plants increased and the proline content decreased after drought treatment.These data further verified that the overexpression of miR190 reduces drought tolerance.Transcriptome resequencing results showed that 1 151 genes of overexpressed miR190 were up-regulated,and 1 836 genes were down-regulated in miRNA overexpres-sion plants.These differentially expressed genes were primarily involved in plant metabolic processes,especially in plant stress.The changes in the content of maize dehydrin gene ZmDHN3 and the expression level of ABA synthe-sis-related gene ZmVP14(NCED)in wild-type and transgenic maize also greatly changed,suggesting that miR190 is involved in maize drought resistance through ABA pathway.
万方数据
维普
