Dynamic Monitoring Saline-alkali Stress Injury at the Maize Seedling Stage Based on Proline Related Indexes
Three maize hybrids(Zhengdan958,Xianyu335,Jidan 27)and their parental lines(Zheng 58/Chang7-2,PH6WC/PH4CV,Si-287/Si-144)were subjected to salt and alkali stress treatments at the seedling stage using 200 mmol/L NaCl and 100 mmol/L Na2CO3,respectively.Seedling growth was monitored,and the Pro content,P5CS,and PDH activities were measured at 10 different time points.Through variance analysis and correlation anal-ysis,the impact of salt-alkali stress on maize was explored,and salt tolerance evaluation indices and time points were selected for comprehensive classification of salt tolerance.The results showed that alkali stress inflicted more severe damage on the plants.Due to the significant phase differentiation in proline-related indicators,the nine stress points were artificially divided into three stages,and the 1st day,3rd day,and 5th day after stress application were identified as suitable time points for evaluating salt-alkali tolerance in maize seedlings.P5CS activity was es-tablished as the evaluation index.Zhengdan958 was identified as a high salt-tolerant variety,Jidan 27 as a high al-kali-tolerant variety,and Si-287,Si-144,and Zheng 58 were classified as high salt/alkali-tolerant inbred lines.This study provides technical support for dynamic monitoring and evaluation of salt-alkali stress damage in maize seedlings.
Maize(Zea mays L.)Salt or alkali stressProline contentP5CS activityPDH activity
万方数据
维普
