首页|玉米茎秆穿刺强度及其相关性状QTL定位

玉米茎秆穿刺强度及其相关性状QTL定位

扫码查看
以PB80(抗穿刺力弱)与PHJ65(抗穿刺力强)为亲本组配的310个重组自交系为材料,采用重测序技术对群体进行基因型分型,结合3个环境下群体茎秆第3节穿刺强度、长度及其直径进行QTL定位.结果表明,共检测到12个玉米茎秆穿刺强度QTLs,单个QTL可解释的表型变异为0.09%-10.67%,其中效应最大的QTL qSRPR2-4在2个环境下重复检测到,认定为主效QTL.节间长度共检测出QTLs 13个,单个QTL可解释的表型变异为2.39%~8.17%.节间直径共检测到QTLs7个,单个QTL可解释的表型变异为2.61%-8.21%.同时从主效QTLqS-RPR2-4区段内筛选出 3个候选基因(AC194970.5_FG005、GRMZM2G028905、GRMZM5G823629),这3个基因均影响茎秆细胞壁纤维素含量.
QTL Mapping of Stem Rind Penetrometer Resistance and Related Traits in Maize
QTL mapping for the rind penetrometer resistance,length and diameter of the third internode in 310 recombination inbred lines derived from a cross between PB80(weak penetration resistance)and PHJ65(strong pene-tration resistance)under three environments were carried out by employed the next-generation sequencing technolo-gy for population genotyping.The results indicated that a total of 12 putative QTLs for rind penetrometer resistance were found,each explaining 0.09%-10.67%of the phenotypic variation.Among them,QTLqSRPR2-4 with the larg-est effect among the 12 QTLs was repeated detected in two environments,and was identified as main effect QTL.A total of 13 putative QTLs for stem length were detected,each accounting for 2.39%-8.17%of the phenotypic varia-tion.Seven QTLs for stem diameter were identified,explaining 2.61%-8.21%of the phenotypic variation.Mean-while,three candidate genes(AC194970.5_FG005,GRMZM2G028905,GRMZM5G823629)were screened from the genomic region of the main effect QTL qSRPR2-4.The three genes affect the cellulose content of stem cell wall.

MaizeStem rind penetrometer resistanceQTL mapping

张凤启、张君、马智艳、张莹莹、齐建双、穆心愿、夏来坤、唐保军、丁勇

展开 >

河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州 450002

安阳市农业科学院,河南安阳 455000

玉米 茎秆穿刺强度 QTL定位

河南省科技攻关项目国家重点研发计划项目河南省玉米产业技术体系建设项目河南省农业科学院科技创新团队项目河南省重点研发专项

2221021102202021YFD1200700HARS-22-02-G22022TD30221111112400

2024

玉米科学
吉林省农业科学院 国家玉米工程技术研究中心(吉林) 国家玉米改良中心 中国农业科技东北创新中心

玉米科学

CSTPCD北大核心
影响因子:1.163
ISSN:1005-0906
年,卷(期):2024.32(8)