Isolation and Identification of a Low Temperature-tolerant Maize Stalk Cellulose-Degrading Fungus
For hastening the process of maize stalk decomposition in northern areas,the research has been con-ducted on isolation and screening of high-efficiency,low-temperature straw cellulose degrading fungi,and study on enzyme production characteristics.In this study,under the condition of 18 ℃,Hutchison medium and plate dilution method were used for primary screening of straw cellulose degrading fungi,combined with Congo red staining.Ac-cording to the degradation of filter paper,enzyme activity analysis and straw degradation rate,the target strain was determined and preliminarily identified through morphological observation and its gene sequence analysis.The re-sults showed that a fungus strain Z1 1 with strong cellulose degradation ability at low temperature was isolated from the soil of low temperature habitat,and its straw degradation ability was 56.3％within 15 days.The enzyme activi-ties of filter paper and CMC were 18.9 U/mL and 54.8 U/mL,respectively.The strain Z11 was preliminarily identi-fied as Aspergillus janus.
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