Whole Genome Identification and Bioinformatics Analysis of Maize HKT Gene Family
A phylogenetic tree was constructed based on the HKT gene family of 14 species,and the gene struc-ture,conserved motifs,and physicochemical properties of the HKT family members of the model crop Arabidopsis Thaliana and three closely related species of maize,sorghum,and rice were deeply analyzed.The alkali-tolerant in-bred line Zheng58 and the alkali-sensitive inbred line Chang7-2 were used as experimental materials to transcrip-tion analysis data of the leaf and root for the seedlings under the stress of 200 mmol/L NaCl or 100 mmol/L Na2CO3 solution at stress 0,1 and 3 days.The results show that the HKT family members of 14 species are divided into two clades in the phylogenetic tree,which may be derived from at least two common ancestral genes.The HKT family has similar gene structure and special gene characteristics.There are 3 members of the maize HKT gene family,which included one type Ⅰ and two type Ⅱ.This paper hypothesizes that having more members of the HKT gene fami-ly contributes to a broader adaptation of plants to salt-alkali environments.Moreover,gene expression analysis un-der salt-alkali treatment showed that the salt-alkali tolerance of Zheng58 and Chang7-2 was directly related to Zm-HKT1;1,indirectly related to ZmHKT2;1 and irrelevant to ZmHKT1;2.This study reveals the regulatory mechanism of HKT gene and has important implications for the comparative genomics analysis of HTK gene families,under-standing plant development and response to abiotic stress.
MaizeHKT gene familyBioinformaticSalt or alkali stressGene expression
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