Identification and Drought Expression Analysis of the Maize LBD/ASL Gene Family
To address the significant limitation on maize yield imposed by drought stress,maize plants have evolved complex physiological and molecular response systems in which transcription factor families play a key role.This study investigates the function of the LBD/ASL gene family in maize and its role in drought stress response.Us-ing bioinformatics methods,45 maize LBD genes were identified from the Ensembl plants database and named ZmLBD1 to ZmLBD45.Phylogenetic analysis categorized these genes into two major groups,Class Ⅰ and Class Ⅱ,which were further subdivided into several subgroups.Gene structure analysis revealed diversity in exon-intron structures within the ZmLBD gene family,identifying 10 conserved motifs.Collinearity analysis indicated that the ZmLBD gene family underwent multiple gene duplication events during evolution,particularly within the Class Ⅰsubfamily,where duplicated genes played a critical role in its expansion.RNA-Seq data expression analysis showed significant changes in the expression patterns of ZmLBD genes across different tissues and developmental stages,es-pecially under drought stress.