玉米LBD/ASL基因家族基因鉴定及抗旱表达分析
Identification and Drought Expression Analysis of the Maize LBD/ASL Gene Family
韩佳蕊 1李昊 2黄馨 3钱步轩 4刘洋 4王梓钰 4吴委林 5张闯4
作者信息
- 1. 延边大学农学院,吉林延吉 133002;吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室,长春 130033
- 2. 吉林省农业科学院/试验地综合服务中心,吉林洮南 137100
- 3. 通化县农民科技教育中心,吉林通化 134100
- 4. 吉林省农业科学院农业生物技术研究所/吉林省农业生物技术重点实验室,长春 130033
- 5. 延边大学农学院,吉林延吉 133002
- 折叠
摘要
通过生物信息学方法,从Ensembl Plants数据库中鉴定出45个玉米LBD基因,并命名为ZmLBD1～ZmLBD45,探讨LBD/ASL基因家族在玉米中的功能及其在干旱胁迫中的作用.系统进化分析将这些基因分为Class Ⅰ和Class Ⅱ两大类,进一步细分为多个亚组.基因结构分析揭示,ZmLBD基因家族在外显子和内含子结构上具有多样性,并鉴定出10个保守基序.共线性分析表明,ZmLBD基因家族在进化过程中经历了多次基因重复事件,尤其在Class Ⅰ亚家族中,重复基因对其扩展起到重要作用.RNA-Seq数据的表达分析显示,ZmLBD基因在不同组织和发育阶段的表达模式尤其在干旱胁迫下发生显著变化.
Abstract
To address the significant limitation on maize yield imposed by drought stress,maize plants have evolved complex physiological and molecular response systems in which transcription factor families play a key role.This study investigates the function of the LBD/ASL gene family in maize and its role in drought stress response.Us-ing bioinformatics methods,45 maize LBD genes were identified from the Ensembl plants database and named ZmLBD1 to ZmLBD45.Phylogenetic analysis categorized these genes into two major groups,Class Ⅰ and Class Ⅱ,which were further subdivided into several subgroups.Gene structure analysis revealed diversity in exon-intron structures within the ZmLBD gene family,identifying 10 conserved motifs.Collinearity analysis indicated that the ZmLBD gene family underwent multiple gene duplication events during evolution,particularly within the Class Ⅰsubfamily,where duplicated genes played a critical role in its expansion.RNA-Seq data expression analysis showed significant changes in the expression patterns of ZmLBD genes across different tissues and developmental stages,es-pecially under drought stress.
关键词
玉米/LBD基因家族/干旱胁迫/转录因子/基因表达/共线性分析Key words
Maize/LBD gene family/Drought stress/Transcription factor/Gene expression/Collinearity analy-sis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据