氮响应转录因子ZmNLP5影响玉米氨基酸代谢的研究
Analysis of Nitrogen-Responsive Transcription Factor ZmNLP5 Affecting Amino Acid Metabolism in Maize
葛敏 1王元琮 1宁丽华 1周玲 1梁帅强 1邬义波 1赵涵1
作者信息
- 1. 江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室,南京 210014
- 折叠
摘要
前期利用遗传和生理生化实验发现,氮响应转录因子ZmNLP5对玉米氮素吸收利用具有促进作用,但其调控机制尚未解明.基于此,以ZmNLP5基因突变体(zmnlp5)和野生型(WT)植株为研究材料,测定充足氮(SN)和低氮(DN)供给下供试材料地上和地下部20种游离氨基酸的含量,从而进一步解析ZmNLP5影响玉米氮代谢的生理机制.结果表明,SN和DN条件下,相对于WT植株,zmnlp5植株地上和地下组织中多种氨基酸(40％～70％)含量显著降低,下降范围为6.08％～77.70％;氨基酸总量也显著降低,下降范围为17.91％～45.82％.此外,发现ZmNLP5对地下组织氨基酸代谢的影响比对地上组织的大,并且ZmNLP5基因的功能在低氮环境下更加显著.
Abstract
Previous genetic and physiological experiments have demonstrated that the transcription factor ZmN-LP5 can improve nitrogen uptake and assimilation in maize,however,the underlying regulation mechanism is un-clear.In this study,twenty free amino acid contents were measured in the shoots and roots of zmnlp5 and wild type(W22)plants under hydroponic culture on sufficient nitrogen(SN)solution and deficient nitrogen(DN)solution.When compared with WT plants,a majority of amino acids(40％-70％)in the zmnlp5 mutant shoots and roots were significantly decreased under SN and DN condition,with approximately 6.08％and 77.70％decreases.The total amino acid content also significantly decreased,with reductions ranging from 17.91％to 45.82％.In addition,zmn-lp5 plants exhibited stronger mutant phenotype in root tissues,especially under deficient nitrogen conditions.
关键词
玉米/氮素/转录因子/氮响应/氨基酸Key words
Maize/Nitrogen/Transcription factor/Nitrogen response/Amino acid引用本文复制引用
出版年
2024
NETL
NSTL
万方数据