Analysis of Nitrogen-Responsive Transcription Factor ZmNLP5 Affecting Amino Acid Metabolism in Maize
Previous genetic and physiological experiments have demonstrated that the transcription factor ZmN-LP5 can improve nitrogen uptake and assimilation in maize,however,the underlying regulation mechanism is un-clear.In this study,twenty free amino acid contents were measured in the shoots and roots of zmnlp5 and wild type(W22)plants under hydroponic culture on sufficient nitrogen(SN)solution and deficient nitrogen(DN)solution.When compared with WT plants,a majority of amino acids(40％-70％)in the zmnlp5 mutant shoots and roots were significantly decreased under SN and DN condition,with approximately 6.08％and 77.70％decreases.The total amino acid content also significantly decreased,with reductions ranging from 17.91％to 45.82％.In addition,zmn-lp5 plants exhibited stronger mutant phenotype in root tissues,especially under deficient nitrogen conditions.
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