玉米逆境胁迫响应因子ZmDof21的克隆及功能分析
Cloning and Functional Analysis of Maize Stress Response Factor ZmDof21
曹丽茹 1王国瑞 2马晨晨 1叶飞宇 1梁小菡 1张新 1鲁晓民1
作者信息
- 1. 河南省农业科学院粮食作物研究所,郑州 450002;神农种业实验室,郑州 450002
- 2. 河南省农业科学院粮食作物研究所,郑州 450002
- 折叠
摘要
利用实验室前期的玉米自交系B104苗期干旱-复水转录组获得1个响应干旱胁迫的Dof基因Zm-Dof21.生信分析显示,ZmDof21包含有1个Dof结构域,且ZmDof21定位在细胞核中.启动子分析显示,ZmDof21启动子区域包含有激素响应元件和胁迫响应元件.荧光定量结果显示,ZmDof 21受到干旱、高温、NaCl和ABA处理的诱导表达,且ZmDof21的表达量与玉米的抗旱性存在正相关关系,干旱胁迫下ZmDof21的表达量在抗旱性强的玉米自交系郑8713的表达量显著高于旱敏感材料B73中的表达量.过表达ZmDof21可以提高转基因拟南芥植株在干旱胁迫下的SPAD含量、叶片相对含水量,降低离子渗透率,同时提高脯氨酸含量及超氧化物歧化酶和过氧化物酶酶活性,从而提高植物的抗旱性.
Abstract
This study utilized drought transcriptome data from early maize seedlings(B 104)in the laboratory to obtain a Dof gene,ZmDof21,which responds to drought stress.Bioinformatics analysis showed that ZmDof21 con-tains a Dof domain and is located in the nucleus.Promoter analysis indicated that the ZmDof21 promoter region con-tains hormone responsive elements and stress responsive elements.qRT-PCR results showed that ZmDof21 was in-duced to express under drought,high temperature,NaCl,and ABA treatments,and the expression level of ZmDof21 was positively correlated with maize drought resistance.The expression level of ZmDof21 under drought stress was significantly higher in the drought resistant maize inbred line Zheng8713 compared to the drought sensitive material B73.Overexpression of ZmDof21 can increase the chlorophyll content,relative leaf water content,and decrease ion permeability of transgenic Arabidopsis plants under drought stress,while increasing proline content and superoxide dismutase activity(SOD)and peroxisome activity(POD),thereby improving drought resistance.
关键词
玉米/Dof转录因子/亚细胞定位/干旱/拟南芥Key words
Maize/Dof transcription factor/Subcellular localization/Drought/Arabidopsis thaliana引用本文复制引用
出版年
2024
NETL
NSTL
万方数据