Cloning and Functional Analysis of Maize Stress Response Factor ZmDof21
This study utilized drought transcriptome data from early maize seedlings(B 104)in the laboratory to obtain a Dof gene,ZmDof21,which responds to drought stress.Bioinformatics analysis showed that ZmDof21 con-tains a Dof domain and is located in the nucleus.Promoter analysis indicated that the ZmDof21 promoter region con-tains hormone responsive elements and stress responsive elements.qRT-PCR results showed that ZmDof21 was in-duced to express under drought,high temperature,NaCl,and ABA treatments,and the expression level of ZmDof21 was positively correlated with maize drought resistance.The expression level of ZmDof21 under drought stress was significantly higher in the drought resistant maize inbred line Zheng8713 compared to the drought sensitive material B73.Overexpression of ZmDof21 can increase the chlorophyll content,relative leaf water content,and decrease ion permeability of transgenic Arabidopsis plants under drought stress,while increasing proline content and superoxide dismutase activity(SOD)and peroxisome activity(POD),thereby improving drought resistance.
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