摘要
目的 分析赖氨酸特异性去甲基化酶1(LSD1)和压电型机械敏感离子通道组件 1(PIEZO1)在口腔癌中表达及与预后转归的关系.方法 选取2013 年1 月—2018 年3 月联勤保障部队第九二〇医院口腔外科收治的口腔癌患者113 例,采用免疫组织化学法检测术中留取口腔癌组织和癌旁组织中LSD1、PIEZO1 表达;分析口腔癌组织LSD1、PIEZO1 表达与临床病理特征的关系;根据口腔癌组织 LSD1、PIEZO1 表达情况分为 LSD1 阳性表达组、PIEZO1阳性表达组、LSD1 阴性表达组、PIEZO1 阴性表达组;采用Kaplan-Meier法绘制LSD1、PIEZO1 阳性/阴性表达口腔癌患者生存曲线,Cox回归分析影响口腔癌患者预后转归的因素.结果 与癌旁组织比较,口腔癌组织中LSD1、PIEZO1阳性表达率升高(χ2/P =47.684/<0.001、43.929/<0.001).LSD1、PIEZO1 在分化程度低、浸润深度>5 mm、TNM分期Ⅲ期、有淋巴结转移患者中阳性表达率升高(LSD1:χ2/P =5.815/0.016、6.669/0.010、8.145/0.004、10.879/0.001,PIEZO1:χ2/P =6.136/0.013、5.796/0.016、6.771/0.009、8.116/0.004).113 例口腔癌患者 5 年总生存率为56.64%(64/113).Kaplan-Meier生存曲线分析显示,LSD1 阳性表达组、PIEZO1 阳性表达组 5 年总生存率分别低于LSD1 阴性表达组、PIEZO1 阴性表达组(χ2/P =12.097/0.001、9.795/0.002).低分化、浸润深度>5 mm、TNM分期Ⅲ期、有淋巴结转移和 LSD1、PIEZO1 阳性表达为影响口腔癌患者预后转归的独立危险因素[OR(95%CI)= 3.131(1.129～8.679)、4.011(1.426～11.283)、5.100(1.274～20.417)、8.357(1.800～38.795)、3.623(1.059～12.395)、3.454(1.191～10.019)].结论 口腔癌组织LSD1、PIEZO1 高表达,与分化程度、浸润深度、TNM分期、淋巴结转移和预后转归有关,可能成为口腔癌患者预后转归评估的生物标志物.
Abstract
Objective To analyze the expression significance of lysine specific demethylase 1(LSD1)and piezoe-lectric mechanosensitive ion channel component 1(PIEZO1)in oral cancer and their relationship with prognosis.Methods One hundred and thirteen patients with oral cancer admitted to the Department of Stomatology at the 920th Hospital of the Joint Logistics Support Force from January 2013 to March 2018 were selected.Immunohistochemical methods were used to detect the expression of LSD1 and PIEZO1 in the intraoperative and adjacent oral cancer tissues;Analyze the relationship between the expression of LSD1 and PIEZO1 in oral cancer tissue and clinical pathological characteristics;According to the expression of LSD1 and PIEZO1 in oral cancer tissue,it is divided into LSD1 positive expression group,PIEZO1 positive ex-pression group,LSD1 negative expression group,and PIEZO1 negative expression group;The Kaplan Meier method was used to plot the survival curves of oral cancer patients with positive/negative expression of LSD1 and PIEZO1,and Coxre-gression analysis was used to analyze the factors affecting the prognosis of oral cancer patients.Results Compared with adjacent tissues,the positive expression rates of LSD1 and PIEZO1 are increased in oral cancer tissues(χ2/P= 47.684/<0.001,43.929/<0.001).LSD1 and PIEZO1 have increased positive expression rates in patients with low differentiation,infil-tration depth>5 mm,TNM stage Ⅲ,and lymph node metastasis(LSD1:χ2/P=5.815/0.016,6.669/0.010,8.145/0.004,10.879/0.001,PIEZO1:χ2/P=6.136/0.013,5.796/0.016,6.771/0.009,8.116/0.004).The 5-year overall survival rate of 113 oral cancer pa-tients was 56.64％(64/113).Kaplan Meier survival curve analysis showed that the overall5-year survival rates of LSD1 posi-tive expression group and PIEZO1 positive expression group were lower than those of LSD1 negative expression group and PIEZO1 negative expression group,respectively(χ2/P=12.097/0.001,9.795/0.002).Low differentiation,infiltration depth>5 mm,TNM stage Ⅲ,lymph node metastasis,and positive expression of LSD1 and PIEZO1 are independent risk factors af-fecting the prognosis of oral cancer patients[OR(95％CI)=3.131(1.129-8.679),4.011(1.426-11.283),5.100(1.274-20.417),8.357(1.800-38.795),3.623(1.059-12.395),3.454(1.191-10.019)].Conclusions The high expression of LSD1 and PIEZO1 in oral cancer tissue is related to differentiation degree,infiltration depth,TNM staging,lymph node metastasis,and progno-sis,and may become biomarkers for evaluating the prognosis of oral cancer patients.
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