Objective To investigate the correlation between peripheral blood soluble urokinase type plasminogen activator receptor(suPAR),tyrosine kinase/signaling and transcription activating factor(JAK/STAT)signaling pathways and airway remodeling in children with bronchial asthma(BA).Methods One hundred and six children with BA admitted to the Respiratory and Critical Care Medicine Department of Gaochang District People's Hospital in Turpan City from January 2021 to January 2023 were selected as the study subjects(BA group).According to the asthma grading criteria,they were divided into intermittent subgroups(n=58),mild subgroups(n=29),and moderate to severe subgroups(n=19).106 healthy children who underwent physical examinations during the same period were selected as the healthy control group.Enzyme linked immunosorbent assay(ELISA)was used to measure serum suPAR levels,while real-time fluorescence quantitative PCR was used to measure serum JAK and STAT levels.Compare the serum levels of suPAR,JAK,STAT,lung function,and airway remodeling among different groups of children;Analyze the relationship between serum suPAR levels,JAK/STAT,lung function,and airway remodeling in children with BA.Results Compared with the healthy control group,the serum levels of suPAR,JAK,STAT,the ratio of airway wall thickness to airway outer diameter(T/D),and the percentage of total airway wall area to total airway area(WA%)in the BA group were all increased(t/P=20.572/<0.001,16.640/<0.001,16.182/<0.001,14.414/<0.001,19.359/<0.001),and the percentage of forced expiratory volume(FEV1)to expected value at the first second(FEV1%)The percentage of FEV1 to forced lung capacity(FEV1/FVC)decreased(t/P= 22.796/<0.001,15.559/<0.001);In the intermittent state subgroup,mild subgroup,and moderate to severe subgroup of BA patients,serum levels of suPAR,JAK,STAT,T/D,and WA%increased sequentially(F/P=23.667/<0.001,52.475/<0.001,30.306/<0.001,76.897/<0.001,62.594/<0.001),while FEV1%,FEV1/FVC decreased sequentially(F/P=99.545/<0.001,91.936/<0.001);Pear-son correlation analysis showed that serum levels of suPAR,JAK,and STAT were significantly negatively correlated with FEV1%and FEV1/FVC(suPAR:r/P=-0.467/<0.001,-0.424/<0.001,JAK:r/P=-0.601/<0.001,-0.560/<0.001,STAT:r/P=-0.458/<0.001,-0.412/<0.001),and were correlated with T/D WA%showed a significant positive correlation(suPAR:r/P=0.427/<0.001,0.411/<0.001,JAK:r/P=0.541/<0.001,0.455/<0.001,STAT:r/P=0.477/<0.001,0.484/<0.001),and FEV1%,FEV1/FVC were significantly negatively correlated with T/D and WA%(T/D:r/P=-0.627/<0.001,-0.546/<0.001,WA%:r/P=-0.590/<0.001,-0.504/<0.001).Conclusion The high expression of suPAR and activation of JAK/STAT pathway may be related to the occurrence and development of BA,and the levels of suPAR and JAK/STAT pathway are closely related to airway remodeling in children with BA,which is expected to become new targets for the diagnosis and treatment of BA.
Bronchial asthmaAirway remodelingSoluble urokinase plasminogen activator receptorTyrosine ki-nase/signal transducer and activator of transcriptionCorrelation
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