Objective To study the correlation and clinical significance between the expression of EMSY transcrip-tion inhibitor(EMSY),p53 induced death domain protein 1(PIDD),and homologous recombination repair genes in non-small cell lung cancer(NSCILC)tissues.Method Eighty patients with NSCLC were treated by the Respiratory and Critical Care Medicine Department of the First Affiliated Hospital of Hebei North University from January 2022 to April 2023.Real time fluorescent quantitative PCR was used to detect the expression of EMSY,PIDD and homologous recombination repair gene human breast cancer susceptibility gene 1(BRCA1),excision repair cross complementary gene 1(ERCC1),ribonucleotide re-ductase subunit 1(RRM1)in cancer tissues and adjacent tissues.Pearson correlation analysis of the correlation between EMSY,PIDD expression and homologous recombination repair gene expression;Analyze the relationship between EMSY,PIDD expression and clinical pathological parameters related to NSCLC,and their value in the diagnosis of NSCLC.Results The relative expression levels of EMSY,PIDD,BRCA1,ERCC1,and RRM1 mRNA in NSCLC cancer tissues were higher than those in adjacent tissues(t/P=30.176/<0.001,27.821/<0.001,25.075/<0.001,16.680/<0.001,25.610/<0.001).The ex-pression of EMSY and PIDD mRNA in NSCLC cancer tissue is positively correlated with BRCA1,ERCC1,and RRM1 mRNA(r/P=0.654/<0.001,0.712/<0.001,0.584/<0.001;0.724/<0.001,0.661/<0.001,0.563/<0.001).The expression of EMSY and PIDD mRNA in low differentiation,lymph node metastasis,and TNM stage Ⅲ NSCLC cancer tissues was higher than that in high differentiation,no lymph node metastasis,and TNM stage Ⅰ-Ⅱ NSCLC cancer tissues(t/P=13.693/<0.001,13.380/<0.001,12.197/<0.001;10.289/<0.001,11.130/<0.001,9.405/<0.001).The AUC of EMSY,PIDD mRNA,and two combined di-agnoses for NSCLC were 0.834,0.802,and 0.906,respectively.The AUC of the two combined diagnoses was greater than that of a single indicator(Z=4.751,5.257,P<0.001).Conclusion The increased expression of EMSY and PIDD in NSCLC cancer tissue is related to the expression of homologous recombination repair genes and adverse clinical and pathological features.The combination of the two is helpful for the diagnosis of NSCLC.
Non-small cell lung cancerEMSYP53 induced death domain protein 1Homologous recombination repair geneDiagnosis
维普
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