Objective To investigate the relationship between serum microRNA-181a-5p and miR-132-5p levels and the 3-year prognosis of patients with chronic glomerulonephritis.Methods One hundred and twenty-eight patients with chronic glomerulonephritis admitted to the Nephrology Department of the 901 Hospital of the Chinese People's Liberation Army Joint Logistics Support Force from January 2018 to April 2020 were selected as the chronic glomerulonephritis group(nephritis group)and 130 healthy individuals who underwent physical examinations were selected as the healthy control group(control group).Detect two groups of renal function indicators,serum miR-181a-5p,and miR-132-5p levels;Analyze the correlation between serummiR-181a-5p,miR-132-5p and renalfunction indicators,identify factors affecting poorprogno-sis within 3 years in patients with chronic glomerulonephritis,and evaluate the value of serum miR-181a-5p,miR-132-5p,and their combination in predicting poor prognosis within 3 years in patients with chronic glomerulonephritis.Results Com-pared with the control group,the levels of serum miR-181a-5p and miR-132-5p in the nephritis group were significantly re-duced(t/P=47.860/<0.001,75.095/<0.001),while the levels of serum uric acid(UA),blood urea nitrogen(BUN),blood cre-atinine(SCR),and cystatin C(CysC)were significantly increased.The level of glomerular filtration rate(eGFR)was signifi-cantly reduced(t=27.103,16.387,37.145,47.506,28.550,all P<0.001).Serum miR-181a-5p is positively correlated with miR-132-5p levels,while serum miR-181a-5p and miR-132-5p are negatively correlated with UA,BUN,Scr,CysC levels,and posi-tively correlated with eGFR levels(r=0.572,-0.506,-0.514,-0.493,-0.627,0.605,-0.498,-0.546,-0.481,-0.609,0.612,all P<0.001).Compared with the subgroup with good prognosis,the subgroup with poor prognosis showed a significant in-crease in serum UA,BUN,SCr,and CysC levels,while eGFR,serum miR-181a-5p,and miR-132-5p levels significantly de-creased(t=6.730,3.596,10.629,7.760,12.657,8.881,13.932,all P<0.001).High levels of UA,BUN,SCr,CysC,eGFR,miR-181a-5p,and miR-132-5p are risk factors for poor prognosis within 3 years in patients with chronic glomerulonephritis[OR(95%CI)=2.239(1.256-3.992),2.768(1.237-6.194),2.173(1.195-3.950),1.806(1.204-2.709),3.022(1.620-5.637),2.565(1.349-4.879),3.350(1.226-9.157)].The area under the curve(AUC)of serum miR-181a-5p,miR-132-5p,and their combined prediction of poor prognosis within 3 years in patients with chronic glomerulonephritis were 0.832,0.801,and 0.904,respec-tively.The AUC predicted by the combination of the two was significantly higher than that predicted by each individual(Z/P=1.714/0.043,2.050/0.020).Conclusions Patients with chronic glomerulonephritis have lower levels of serum miR-181a-5p and miR-132-5p,which are closely related to renal function.The combination of the two can help evaluate the patient's prog-nosis within 3 years.
维普
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