Objective To investigate the expression of spinal protein 2(SPON2)and matrix Gla protein(MGP)in colorectal cancer(CRC)tissue and their relationship with clinical pathological features and prognosis.Method Ninety-four CRC patients underwent surgery who were admitted to the General Surgery Department of Nanjing First Hospital from Janu-ary 2018 to January 2020 were selected.Immunohistochemical staining was used to detect the expression of SPON2 and MGP in cancer and adjacent tissues;Analyze the relationship between the expression of SPON2 and MGP and the clinical pathologicalcharacteristics and prognosis of CRC patients;Multivariate Cox regression analysis of prognostic factors in CRC patients.Results The positive rates of SPON2 in CRC cancer and adjacent tissues were 65.96%(62/94)and 6.38%(6/94),respectively,while the positive rates of MGP were 63.83%(60/94)and 8.51%(8/94),respectively.The positive rates of SPON2 and MGP in CRC cancer tissues were higher than those in adjacent tissues(χ2=72.251,62.298,both P<0.001).The positive rates of SPON2 and MGP in patients with TNM stage Ⅲ,low differentiation,and lymph node metastasis were higher than those in patients with TNM stage Ⅰ-Ⅱ,high differentiation,and no lymph node metastasis,respectively(χ2/P= 9.694/0.002,10.883/0.001,14.100/<0.001,14.785/<0.001,9.731/0.002,12.739/<0.001).The 3-year survival rates of SPON2 positive and negative groups were 54.84%(34/62)and 86.67%(26/30),respectively;The 3-year survival rates of MGP posi-tive and negative groups were 53.33%(32/60)and 87.50%(28/32),respectively.The 3-year cumulative survival rate of SPON2 positive group and MGP positive group is lower than that of SPON2 negative group and MGP negative group(χ2=8.966,12.420,P=0.003,<0.001).Tumor TNM stage Ⅲ,low differentiation,lymph node metastasis,SPON2 positive,and MGP positive are independent risk factors affecting the prognosis of CRC patients[HR(95%CI)=1.768(1.226-2.551),1.613(1.223-2.126),1.600(1.167-2.194),1.866(1.324-2.630),1.315(1.151-1.503),all P<0.001].Conclusion Elevated expression of SPON2 and MGP in CRC is associated with adverse clinical and pathological features of CRC,and they are new tumor markers for evaluating the prognosis of CRC patients.
Colorectal cancerSpinal protein 2Matrix Gla proteinPrognosis
维普
万方数据
