Objective To investigate the effect of curcumin on the expression of IL-36α and IL-36γ in colitis in-duced by trinitrobenzenesulfonic acid(TNBS)in rats.Methods The experiment was conducted in May 2022 at the Medical Experimental Animal Center of Guangxi Medical University.Forty male SD rats were randomly divided into a normal control group,a model group,a curcumin group,and a sulfasalazine group,with 10 rats in each group.The model group,curcumin group,and sulfasalazine group of rats were prepared with TNBS ethanol solution enema to create rat colitis models,while the normal control group was given an equal volume of 0.9%sodium chloride solution enema.The curcumin group and sul-fasalazine group were orally administered with curcumin and sulfasalazine solutions of 100 mg·kg-1·d-1 from the second day of modeling.The normal control group and model group were orally administered with an equal volume of 0.9%sodium chloride solution daily;After 7 days of administration,the rats were euthanized and serum and colon mucosa were collected.The body weight,disease activity index(DAI),and intestinal mucosal injury index(CMDI)of each group of rats were com-pared;ELISA method was used to detect the serum levels of IL-36α and IL-36γ in rats,and immunohistochemical method was used to measure the protein expression of IL-36α and IL-36γ in intestinal mucosal tissue.Results Compared with the normal control group,the DAI and CMDI scores of the model group increased significantly(P<0.01);The levels of serum IL-36α and IL-37 both increased(P<0.01);Compared with the model group,the DAI,CMDI scores,and serum IL-36αand IL-36γ levels in both the curcumin group and the sulfasalazine group decreased,and the curcumin group was lower than the sulfasalazine group,with statistically significant differences(F=3.531,3.842,24.503,36.435,P<0.001);Compared with the normal control group,the protein expression levels of IL-36 and IL-37 in the model group increased(P<0.05);Compared with the model group,the protein expression levels of IL-36α and IL-36γ in the colon mucosa of the curcumin group and the sulfasalazine group decreased,and the curcumin group was lower than the sulfasalazine group,with statistical signifi-cance(F=3.461,3.524,P<0.001).Conclusion Curcumin can significantly inhibit the expression of IL-36α and IL-36γ in se-rum and colon mucosa of experimental colitis rats.
万方数据
