Objective To investigate the relationship between the expression of long coding RNA FOXD2 adjacent opposite strand RNA 1(lncRNA FOXD2-AS1)and miR-1913 in non-small cell lung cancer(NSCLC)tissue and survival peri-od.Methods A total of 100 NSCLC patients admitted to Binzhou Medical College Yantai Affiliated Hospital Cancer Center from November 2017 to November 2019 were regarded as the study subjects.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was applied to determine the expression levels of lncRNA FOXD2-AS1 and miR-1913 in tissues;Pearson method was applied to analyze the correlation between lncRNA FOXD2-AS1 and miR-1913 expression levels in NSCLC tissues;Kaplan-Meier method was applied to analyze the relationship between lncRNA FOXD2-AS1,miR-1913 ex-pression and prognosis in NSCLC tissues;Cox was applied to analyze the influencing factors of prognosis in NSCLC pa-tients.Results Compared with the adjacent tissues,the expression level of lncRNA FOXD2-AS1 in NSCLC tissues in-creased and the expression level of miR-1913 decreased(t=11.439,17.709,P＜0.001).Target Scan Human website predicts that there is a binding site between lncRNA FOXD2-AS1 and miR-1913.The expression level of lncRNA FOXD2-AS1 in NSCLC was negatively correlated with miR-1913(r=-0.406,P＜0.001).Compared with the survival subgroup,the expres-sion level of lncRNA FOXD2-AS1 in the death subgroup increased and the expression level of miR-1913 decreased(t= 6.973,6.307,P＜0.001).The high expression rate of lncRNA FOXD2-AS1 in NSCLCpatients with TNM stageⅢ+Ⅳ,lymph node metastasis and low differentiation was higher than that in NSCLC patients with TNM stage Ⅰ+Ⅱ(χ2 =5.962,P= 0.015),no lymph node metastasis(χ2 =5.104,P=0.024)and moderate/high differentiation(χ2 =6.150,P=0.013).The low ex-pression rate of miR-1913 in NSCLC patients with TNM stage Ⅲ+Ⅳ,lymph node metastasis and low differentiation was higher than that in NSCLC patients with TNM stage Ⅰ+Ⅱ(χ2 =11.457,P=0.001),no lymph node metastasis(χ2 =7.695,P=0.006)and moderate/high differentiation(χ2 =11.349,P=0.001).The 3-year survival rate of NSCLC patients with high ex-pression of lncRNA FOXD2-AS1 was lower than that of patients with low expression of lncRNA FOXD2-AS1(χ2 =7.830,P=0.005),and that of patients with high expression of miR-1913 was higher than that of patients with low expression of miR-1913(χ2 =6.125,P=0.013).Multivariate Coxanalysis showed that high miR-1913 was a protective factor for the progno-sis of NSCLCpatients,the high levelof lncRNA FOXD2-AS1,TNM Ⅲ+Ⅳ stage,lymph node metastasis and lowdegree of differentiation are the risk factors for the prognosis of NSCLC patients[OR(95%CI)= 0.864(0.774-0.964),2.544(1.481-4.370),3.647(1.614-8.242),2.544(1.481-4.370),2.986(1.361-6.553)].Conclusion The expression level of ln-cRNA FOXD2-AS1 increases and the expression level of miR-1913 decreases in NSCLC tissue,both of which are related to the prognostic survival period of patients.
Non-small cell lung cancerLncRNA FOXD2-AS1Micro RNA-1913Survival period
维普
万方数据
