Objective To investigate the expression and clinical significance of Cullin4B E3 ubiquitin ligase(CUL4B)and human transient receptor potential M2(TRPM2)in pancreatic cancer.Methods Eighty-six patients with pan-creatic cancer who were diagnosed and treated in author's hospital from March 2019 to March 2020 were selected.Immuno-histochemistry was used to detect the expression of CUL4B and TRPM2 proteins in tissues.Real time fluorescence quantita-tive PCRwas used to detect the expression of CUL4Band TRPM2 mRNA in tissues.Spearman rank correlation analysis was used to analyze the relationship between CUL4B and TRPM2 protein expression.Kaplan-Meier curve analysis was used to analyze the impact of CUL4B and TRPM2 expression on patient prognosis.Coxproportional hazards model was used to an-alyze the prognostic factors of pancreatic cancer.Results The positive rate of CUL4B and TRPM2 protein and the relative expression of mRNA in pancreatic cancer tissues were higher than those in adjacent tissues,and the difference was statisti-cally significant(χ2/t=70.245,60.224,15.741,10.976,P＜0.001).The expression of CUL4B was positively correlated with TR-PM2 in pancreatic cancer(r=0.720,P＜0.001).The positive rates of CUL4B and TRPM2 protein in TNM stage Ⅱ B-Ⅲ,lymph node metastasis pancreatic cancer were higher than those in stage Ⅰ-Ⅱ A,and no lymph node metastasis(χ2 = 16.511,14.834,15.576,15.007,P＜0.001).The 3-year overall survival rates of the CUL4B positive and negative groups were 9.68%(6/62)and 41.67%(10/24),respectively.The 3-year overall survival rates of the TRPM2 positive and negative groups were 8.33%(5/60)and 42.31%(11/26),respectively.The 3-year cumulative survival rate of patients in the CUL4B positive group and TRPM2 positive group was lower than that in the CUL4B negative group and TRPM2 negative group,and the difference was statistically significant(Log-Rank χ2 =9.933,11.102,P=0.002,0.001).TNM stage,lymph node metastasis,CUL4B,TRPM2 were independent factors affecting the prognosis of pancreatic cancer[HR(95%CI)=1.781(1.199-2.646),1.962(1.172-3.285),2.482(1.445-4.263),1.733(1.223-2.457)].Conclusion The expression of CUL4B and TRPM2 in pancre-atic cancer are elevated,which are related to the poor clinicopathological characteristics of pancreatic cancer and are prog-nostic tumor markers of pancreatic cancer.
维普
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