Objective To explore the correlation between the expression of long non-coding RNA cancer suscepti-bility candidate 2(lncRNA CASC2),microRNA-532-3p(miR-532-3p)in breast cancer(BC)tissue and the survival of patients within 5 years after surgery.Methods A total of 127 BC patients admitted to Department of General Surgery,the Fifth Peo-ple's Hospital of Datong City from January 2015 to June 2018 were selected,BC tissues and para-cancer normal tissues were collected during the operation,and the expressions of lncRNA CASC2 and miR-532-3p in BCtissues and para-cancer normal tissues were detected by fluorescence quantitative PCR;the BC patients were followed up for 5 years after surgery,the sur-vival and death of the patients within 5 years were recorded.The expressions of lncRNA CASC2 and miR-532-3p in para-cancer normal tissues and BC tissues,the expression differences of lncRNA CASC2 and miR-532-3p in BC tissues in differ-ent clinicopathological characteristics,the clinicopathological characteristics and the expressions of lncRNA CASC2 and miR-532-3p in BC tissues of survival group and death group were compared.Analyze the correlation between the expression of lncRNA CASC2 and miR-532-3p in BC tissue,the relationship between the expression of lncRNA CASC2 and miR-532-3p in BC tissue and postoperative 5-year survival,the factors affecting postoperative 5-year survival of BC patients,and the predictive value of lncRNA CASC2 and miR-532-3p for postoperative 5-year survival of BC patients.Results The expres-sion level of lncRNA CASC2 in BC tissues was significantly lower than that in para-cancer normal tissues,and the expres-sion level of miR-532-3p in BC tissues was significantly higher than that in para-cancer normal tissues(t/P=38.239/＜0.001,49.406/＜0.001).The proportion of patients with tumor diameter≥2 cm,TNM stage Ⅲ,low differentiation of tumors and lymph node metastasis in lncRNA CASC2 low expression group were higher than in the high expression group,while the miR-532-3p low expression group was lower than that in the high expression group(lncRNA CASC2:χ2/P=17.361/＜0.001,17.052/＜0.001,14.694/＜0.001,13.173/＜0.001,miR-532-3p:χ2/P=10.733/0.001,9.813/0.002,10.134/0.001,7.444/0.006).127 BC patients were followed up for 5 years after surgery,without loss of follow-up,99 cases survived(survival group),28 cases died(death group).The proportion of patients with tumor diameter≥2 cm,TNM stage Ⅲ,low differentiation of tumors,lymph node metastasis and miR-532-3p expression level in the death group were higher than in the survival group,while the lncRNA CASC2 expression level in the death group was lower than in the survival group[χ2(t)/P= 5.211/0.022,27.149/＜0.001,27.990/＜0.001,4.590/0.032,19.155/＜0.001,10.818/＜0.001].LncRNA CASC2 was negatively correlated with miR-532-3p expression in BC tissues(r/P=-0.561/＜0.001).The 5-year overall survival rate of BC patients after surgery in the lncRNA CASC2 high expression group was 89.23%(58/65)higher than that in the lncRNA CASC2 low expression group 66.13%(41/62)(χ2/P=9.854/0.002);the 5-year overall survival rate of BC patients after surgery in the miR-532-3p high ex-pression group was 65.57%(40/61)lower than that in the miR-532-3p low expression group 89.39%(59/66)(χ2/P=10.466/0.001).Tumor diameter≥2 cm,TNM stage Ⅲ,low differentiation of tumors,lymph node metastasis,lncRNA CASC2 low expression,and miR-532-3p high expression were all independent risk factors affecting the survival of BC patients within 5 years after surgery[HR(95%CI)=2.255(1.192-4.263),2.143(1.252-3.666),3.089(1.386-6.887),2.219(1.223-4.026),2.606(1.174-5.788),2.855(1.592-5.120)].The area under curve(AUC)of lncRNA CASC2,miR-532-3p,their combined in predic-ting postoperative 5-year survival of BC patients was 0.840,0.852 and 0.908,respectively,AUC predicted by the combination of the two methods was higher than that predicted by lncRNA CASC2 and miR-532-3p separately(Z/P= 2.246/0.025,2.033/0.042).Conclusion The expression of lncRNA CASC2 is down-regulated and the expression of miR-532-3p is up-reg-ulated in BC tissues,and the changes in BC patients who died within 5 years after surgery are more significant than those who survived.The expressions of both are correlated with clinicopathological characteristics,and are of high value in pre-dicting the survival of BC patients within 5 years after surgery.
Breast cancerLong non-coding RNA cancer susceptibility candidate 2MicroRNA-532-3pSurvival within 5 years after surgeryCorrelation
维普
万方数据
