Objective To analyze the correlation between the expression of serum silence-information regulatory factor(SIRT1),transforming growth factor β1 protein(TGF-β1)and dysfunction indexand pain score in patients with degen-erative lumbar spinal stenosis(DLSS).Methods A total of 86 DLSS patients admitted to the Trauma and Spinal Department of Fenyang Hospital in Shanxi Province from October 2020 to October 2023 were selected as the DLSS group.According to the Oswestry Low Back Pain Dysfunction Index(ODI),patients were divided into mild to moderate dysfunction subgroup(n=50)and severe dysfunction subgroup(n=36).In the same period,85 healthy subjects in hospital were used as healthy control group.The clinical data of 2 groups were collected,and the total adipose multifidus cross-sectional area(TFCSA)and total multifidus cross-sectional area(TCSA)of all subjects were measured,and the ratio of TFCSA/TCSA was calculat-ed.Serumlevels of SIRT1,TGF-β1,cortisol and adrenocorticotropin(ACTH)were detected by enzyme-linked immunosorbent assay(ELISA).Low back pain dysfunction was assessed by ODI.Visual analogue pain scale(VAS)was used to evaluate the pain degree of the subjects.Spearman method was used to analyze the correlation between serum SIRT1,TGF-β1 level and ODI index and VAS score.The influencing factors of DLSS were analyzed by Logistic regression.Results The levels of TFCSA,TFCSA/TCSA and serum SIRT1,TGF-β1 in DLSS group were lower than those in healthy control group(t/P= 8.696/＜0.001,6.669/＜0.001,38.323/＜0.001,22.313/＜0.001).Cortisol,ACTH,ODI index and VAS scores were higher than those of healthy control group(t/P=57.280/＜0.001,58.495/＜0.001,48.583/＜0.001,53.355/＜0.001).Serum levels of SIRT1,TGF-β1,TFCSA and TFCSA/TCSA were decreased in the severe dysfunction subgroup compared with the mild to moderate dysfunction subgroup(t/P=13.834/＜0.001,4.684/＜0.001,3.520/0.001,5.418/＜0.001).Cortisol,ACTH,ODI and VAS scores were increased(t/P=6.168/＜0.001,2.355/0.021,15.784/＜0.001,11.004/＜0.001).Correlation analysis showed that serum SIRT1 and TGF-β1 levels were negatively correlated with ODIindexand VASscore(r=-0.534,-0.577,-0.602,-0.556,all P＜0.001).High levels of serum SIRT1 and TGF-β1 were protective factors for DLSS[OR(95%CI)=0.687(0.491-0.961),0.547(0.401-0.746)].High cortisol,high ACTH,high ODI and high VAS score were the risk factors for DLSS[OR(95%CI)=2.468(1.200-5.077),2.673(1.162-6.148),3.345(1.165-9.602),3.123(1.457-6.694)].Conclusion Serum SIRT1 and TGF-β1 levels decrease in DLSS patients,which are negatively correlated with ODI indexand VAS score,and are influencing fac-tors for DLSS.
Lumbar spinal stenosis,degenerativeSilent information regulator1Transforming growth factor-β1 pro-teinDysfunction indexPain score
维普
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