Objective To investigate the expression changes in Rho-related associated-coil forming protein kinase 2(ROCK2)and renalase in serum of patients with idiopathic membranous nephropathy(IMN)and their diagnostic value for IMN.Methods From January 2019 to November 2020,120 patients with IMN admitted to the Nephrology Department of Yiling Hospital in Hebei Province were selected as the IMN group,and another 120 volunteers who underwent physical ex-amination in the hospital during the same period were selected as healthy control group.Enzyme-linked immunosorbent as-say(ELISA)was used to detect the levels of serum ROCK2 and renalase in patients with IMN,the correlation between ROCK2 and renalase levels and some clinical indicators was analyzed by Pearson's method;the value of ROCK2,renalase and their combination in the diagnosis of IMN was analyzed by receiver operating characteristic(ROC)curve.Results The levels of ROCK2,renalase in the IMN group were greatly higher than those in healthy control group(t/P=12.837/<0.001,11.066/<0.001).Serum ROCK2 was gradually increased in patients with stageⅠ,Ⅱ,Ⅲand ⅣIMN(F/P=77.154/<0.001);the level of serum renalase in stageⅠ,Ⅱ,andⅢgradually increased,but the level of serum renalase in stageⅣwere low-er than those in stage Ⅲ(F/P= 163.042/<0.001).Alb was negatively correlated with ROCK2 and renalase levels(r/P=-0.302/0.009,-0.402/<0.001).PLA2R antibody,BUN,SCr and 24 h urinary protein were positively correlated with ROCK2 and renalase levels(r=0.336/0.359,0.264/0.284,0.315/0.420,0.320/0.412,0.359/<0.001,0.284/0.010,0.420/<0.001,0.412/<0.001);ROCcurve showed that the AUCof ROCK2 for the diagnosis of IMNwas 0.908,the AUCof renalase for the diagnosis of IMN was 0.907,the AUC of the two combined for the diagnosis of IMN was 0.965,which was greatly higher than that of the two alone(Z=3.597,3.755,P<0.001).Conclusion Both ROCK2 and renalase are highly expressed in the serum of patients with IMN,and the combination of the two has a high value in the diagnosis of IMN.
Idiopathic membranous nephropathyRho-associated coiled-coil forming protein kinase 2NephrinaseDiagnostic value
国家科技期刊平台
NSTL
维普
万方数据
