Objective To investigate the expression of DEAD-boxhelicase 24(DDX24)and thyroid hormone re-ceptor interactor 12(TRIP12)in hepatocellular carcinoma(HCC),and analyze their relationship with epithelial mesenchymal transition(EMT)and clinical prognosis.Methods A retrospective selection was conducted on 96 HCC patients who under-went liver and gallbladder surgery at the 940 Hospital of the Chinese People's Liberation Army Joint Logistics Support Force from February 2019 to January 2021.The expressions of DDX24 and TRIP12 proteins were detected by immunohistochemis-try.The expressions of DDX24 mRNA,TRIP12 mRNA and EMT markers E-cadherin(E-cad)mRNA,N-cadherin(N-cad)mR-NA and TWIST mRNA were detected by real-time fluorescence quantitative PCR Pearson correlation analysis was used to analyze the correlation between DDX24 mRNA,TRIP12 mRNA and EMT indicators.Kaplan-Meier curve was drawn,and Log-Rank test was used to analyze the difference in prognosis of HCC patients between DDX24 mRNA and TRIP12 mRNA high expression group and low expression group.Multivariate Cox proportional hazards model was used to analyze the inde-pendent factors affecting the prognosis of HCC patients.Results The positive rates of DDX24 and TRIP12 in HCC cancer tissues were higher than those in adjacent tissues(x2/P=109.714/＜0.001,108.755/＜0.001).Compared with adjacent tissues,the expressions of DDX24 mRNA,TRIP12 mRNA,N-cad mRNA and TWIST mRNA in HCC cancer tissues were higher,and E-cad mRNA was lower,and the differences were statistically significant(t/P=35.810/＜0.001,48.036/＜0.001,25.015/＜0.001,48.482/＜0.001,38.069/＜0.001).DDX24 mRNA expression was positively correlated with TRIP12 mRNA expression in HCC tissues(r=0.701,P＜0.001).DDX24 mRNA and TRIP12 mRNA were positively correlated with N-cad mRNA and TWIST mRNA,and negatively correlated with E-cad mRNA(r/P=0.723/＜0.001,0.661/＜0.001,0.706/＜0.001,0.745/＜0.001,-0.694/＜0.001,-0.609/＜0.001).DDX24 mRNA and TRIP12 mRNA in HCC tissues of CNLC stage Ⅱ-Ⅲ,poor differentiation and no vascular invasion were higher than those in CNLC stage Ⅰ and moderately and highly differentiated and vascular invasion(DDX24:t/P=10.348/＜0.001,18.474/＜0.001,6.302/＜0.001;TRIP12:t/P=25.661/＜0.001,36.396/＜0.001,14.928/＜0.001).The 3-year overall survival rates of DDX24 mRNA high expression group and low expression group were 39.13％(18/46)and 64.00％(32/50),respectively.There was a significant difference in the overall survival curve between the two groups(Log rank x=7.491,P=0.006).The 3-year overall survival rates of TRIP12 mRNA high expression group and low expression group were 36.17％(17/47)and 67.35％(33/49),respectively.There was a significant difference in the overall survival curve between the two groups(Log rank x2=8.146,P=0.004).CNLC stage Ⅱ-Ⅲ,poor differentiation,vascular invasion,high expression of DDX24 mRNA,and high expression of TRIP12 mRNA were risk factors for the death and prognosis of HCC patients[HR(95％CI)=1.611(1.175-2.209),1.768(1.238-2.526),1.464(1.089-1.968),1.859(1.330-2.599),1.775(1275-2.473)].Conclusion DDX24 and TRIP12 are up-regulated in HCC,which are correlated with EMT markers,CNLC stage Ⅱ-Ⅲ,degree of differentiation and vascular invasion.Both of them are helpful to evaluate the prognosis of HCC patients.
维普
万方数据
